Location: Bioproducts ResearchTitle: Use of sodium alginate coatings to improve bioavailability of liposomes containing DPP-IV inhibitory collagen peptides
|WU, PEIHAN - Jiangnan University|
|CHEN, LING - Jiangnan University|
|CHEN, MAOSHEN - Jiangnan University|
|XU, FEIFEI - Jiangnan University|
|LIU, FEI - Jiangnan University|
|ZHONG, FANG - Jiangnan University|
Submitted to: Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/10/2023
Publication Date: 2/14/2023
Citation: Wu, P., Chen, L., Chen, M., Chiou, B., Xu, F., Liu, F., Zhong, F. 2023. Use of sodium alginate coatings to improve bioavailability of liposomes containing DPP-IV inhibitory collagen peptides. Food Chemistry. 414. Article 135685. https://doi.org/10.1016/j.foodchem.2023.135685.
Interpretive Summary: Collagen peptides have been shown to effectively inhibit enzymes that inactivate hormones, which regulate the blood sugar level. Consequently, the collagen peptides can be used as a possible treatment for diabetes. However, the peptides are easily destroyed in the gastrointestinal tract due to acidic and enzymatic degradation. In this study, we examined the effects of using alginate coatings to protect peptides encapsulated in liposomes from degradation. The alginate coatings improved the encapsulation efficiency of peptides in liposomes. In addition, the coatings improved the controlled-release profiles of the peptides as well as the peptide's stability in the gastrointestinal tract. These results indicated that the alginate coatings can be used to improve the bioavailability of peptides encapsulated in liposomes.
Technical Abstract: Sodium alginate (SA) was used to coat liposomes containing DPP-IV inhibitory collagen peptides to improve their stability and in vitro absorption for intra-oral delivery. The liposomes were characterized using transmission electron microscopy, Fourier transform infrared spectroscopy as well as dynamic light scattering to determine their average diameter, polydispersity index and zeta potential. The entrapment efficiency (EE) and DPP-IV inhibitory activity of the liposomes were also determined. The storage stability of liposomes was determined. Moreover, the vitro stability of liposomes was determined by measuring in vitro release rates and their gastrointestinal stability. Finally, transcellular permeability of liposomes was tested to characterize their permeability in small intestinal epithelial cells. The results showed that the sodium alginate coating increased the diameter, absolute value of zeta potential and EE of liposomes. The sodium alginate-coated liposomes containing collagen peptides (SA-Co-Lip) showed enhanced storage stability, gastrointestinal stability, transcellular permeability and reduced in vitro release rates compared to uncoated liposomes (Co-Lip). SA-Lip are promising carriers for transporting hydrophilic molecules, may be beneficial for improving nutrient absorption and can protect bioactive compounds from being inactivated in the gastrointestinal tract.