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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Animal Health Genomics » Research » Publications at this Location » Publication #397258

Research Project: Strategies to Control Respiratory Diseases of Cattle

Location: Animal Health Genomics

Title: Changes in serum haptoglobin concentration in cattle administered LPS

item TOUZE, ANGELICA - Former ARS Employee
item Chitko-Mckown, Carol

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/15/2022
Publication Date: 8/5/2022
Citation: Touze, A., Chitko-Mckown, C.G. 2022. Changes in serum haptoglobin concentration in cattle administered LPS. In proceedings: 2022 National Veterinary Scholars Symposium, University of Minnesota, Minneapolis, Minnesota. August 4-7, 2022.Abstract. pg. 298.

Interpretive Summary:

Technical Abstract: Haptoglobin (HPT) is an acute phase protein that is produced in the liver. Lipopolysaccharide (LPS) is a component of the cell wall in gram-negative bacteria. Also known as endotoxin, it binds to toll-like receptor 4 (TLR-4) and CD14 on immune cells to initiate an inflammatory response. Lipopolysaccharide can therefore be used to model an inflammatory response similar to when cattle are infected with a complex of viral and bacterial pathogens as found in bovine respiratory disease complex. Previous studies in our laboratory indicated that HPT remained elevated in blood post-administration of LPS, even after clinical signs reverted to normal. Therefore, we designed a study to follow serum concentrations of HPT in LPS-treated cattle over the course of 4 weeks. This study will determine the time required for HPT to return to baseline concentrations post LPS administration. Thirty cattle will be included in our experiment. Fifteen will be given an intravenous injection of LPS (0.25 µg/kg in 1-3 ml saline) and 15 Control animals will receive saline only. Cattle will be observed over the course of 28 days. The data collected will include clinical signs, rectal temperature, respiration rates and HPT concentration measured in serum collected from whole blood. These measurements will be taken prior to LPS treatment (D0) and 24 hours post-treatment (D1) as well as once weekly for 4 weeks (D7, D14, D21, D28). We hypothesize that serum HPT concentration will return to baseline concentration between 7 to 14 days.