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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #397010

Research Project: Mapping Crop Genome Functions for Biology-Enabled Germplasm Improvement

Location: Plant, Soil and Nutrition Research

Title: Regulatory networks governing nitrogen use efficiency in maize and sorghum

item BRAYNEN, JANEEN - Cold Spring Harbor Laboratory
item ZHANG, LIFANG - Cold Spring Harbor Laboratory
item OLSON, ANDREW - Cold Spring Harbor Laboratory
item KUMARI, SUNITA - Cold Spring Harbor Laboratory
item KUMAR, VIVEK - Cold Spring Harbor Laboratory
item REGULSKI, MICHAEL - Cold Spring Harbor Laboratory
item LISERON-MONFILS, CHRISTOPHE - National Research Council - Canada
item KOCHIAN, LEON - University Of Saskatchewan
item BRADY, SIOBHAN - University Of California, Davis
item Ware, Doreen
item GUADINIER, ALLISON - University Of California Berkeley
item ABBITT, SHANE - Corteva Agriscience
item FRANK, MARY - Corteva Agriscience
item SHEN, BO - Corteva Agriscience

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 6/2/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Nitrogen (N) is an essential micronutrient for plants. Maximizing Nitrogen Use Efficiency (NUE) in plants is vital to increase crop production and reduce negative impacts on the environment. In order to explore the gene regulatory network (GRN) that controls the NUE related processes, we have profiled the maize transcriptome in response to N limitation. Based on this profile, we have used yeast one-hybrid (Y1H) technology to systematically map the GRN that governs the genes that are known to be involved in the process of nitrogen uptake, assimilation, utilization, remobilization and transcriptional regulation in maize. We have compared this regulatory network with its counterpart NUE network in Arabidopsis, conducted correlation analysis using expression data and identified key transcription factors (TFs) that regulate maize genes involved in NUE. Our data indicated that 36% of the edges in the network (TF and promoter interactions) were conserved in homologous pairs between Arabidopsis and maize. TFs in EREB, NAC, bZIP, MYB, bHLH, ARF and NLP families are conserved in their regulatory functions in NUE processes. The conservation is more prominent for the genes that regulate nitrate assimilation steps.