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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Animal Health Genomics » Research » Publications at this Location » Publication #396762

Research Project: Strategies to Control Respiratory Diseases of Cattle

Location: Animal Health Genomics

Title: Current and emerging diagnostic approaches to bacterial diseases of ruminants

item LOY, JOHN - University Of Nebraska
item Clawson, Michael - Mike
item ADKINS, PAMELA - University Of Missouri
item MIDDLETON, JOHN - University Of Missouri

Submitted to: Veterinary Clinics of North America
Publication Type: Review Article
Publication Acceptance Date: 10/12/2022
Publication Date: 3/1/2023
Citation: Loy, J.D., Clawson, M.L., Adkins, P.R.F., Middleton, J.R. 2023. Current and emerging diagnostic approaches to bacterial diseases of ruminants. Veterinary Clinics of North America, Food Animal Practice. 39:93-114.

Interpretive Summary:

Technical Abstract: The diagnostic approaches and methods to detect bacterial pathogens in ruminants are discussed, with a focus on cattle. Conventional methods using culture, isolation, and characterization are being replaced or supplemented with new methods. These include molecular diagnostics such as real time PCR and whole genome sequencing. Additionally, methods such as MALDI-TOF mass spectrometry enables rapid identification and enhanced pathogen characterization. These new diagnostic tools can greatly enhance the ability to detect and characterize pathogens, but results and interpretation vary greatly across sample and pathogen types, disease syndromes, assay performance, and other factors. A brief overview of emerging diagnostic methods and their application and interpretation for several clinical diseases is provided.