Temporal profile of neonicotinoid concentrations in cotton, corn, and soybean resulting from insecticidal seed treatments

Authors: WHALEN, ADAM - Mississippi State University; CATCHOT, ANGUS - Mississippi State University; GORE, JEFF - Mississippi State University; STEWART, SCOTT - Mississippi State University; LORENZ, GUS - University Of Arkansas; COOK, DONALD - Mississippi State University; MUSSER, FRED - Mississippi State University; HARRIS, JEFFERY - Mississippi State University; KRISHNAN, NATRAJ - Mississippi State University

Submitted to: Agronomy
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/9/2021
Publication Date: 6/12/2021
Citation: Whalen, A., Catchot, A.L., Gore, J., Stewart, S.D., Lorenz, G.M., Cook, D.R., Musser, F.R., Harris, J.W., Krishnan, N. 2021. Temporal profile of neonicotinoid concentrations in cotton, corn, and soybean resulting from insecticidal seed treatments. Agronomy. 11(6):1200. https://doi.org/10.3390/agronomy11061200.
DOI: https://doi.org/10.3390/agronomy11061200

Interpretive Summary: The neonicotinoids are a widely employed class of insecticides used in row crop agriculture to protect against a broad range of economically important pests. Neonicotinoids have been implicated as a contributing factor to the observed decreases in honey bee populations. It has been suggested that honey bees can be exposed to seed-treated neonicotinoids through pollen and nectar from treated plants. To investigate the uptake and persistence of neonicotinoids in plant tissue and soil, we conducted seed treatment trials with corn, cotton, and soybean planted in Mississippi, Arkansas, and Tennessee during the 2013 and 2014 growing seasons. Leaf tissue was collected and analyzed beginning shortly after emergence until plants began to flower to better understand how neonicotinoid concentrations change in plant tissues over time. The youngest leaf in the terminal of the plant was sampled as an indicator of the neonicotinoid concentrations within the plant. Soil samples were also collected and analyzed for neonicotinoid concentrations at the first and last sampling dates. Overall, there was a approximate 98 - 100% reduction (dependent on plant type and concentration used) in concentrations in the neonicotioids tested by the time that plants reached reproductive stages.reached reproductive growth. The data for neonicotinoid concentrations in the soil were more variable than leaf tissue samples, and the reduction in neonicotinoid concentrations in leaf tissues did not closely correlate with concentrations in the soil. Our results suggest that neonicotinoid insecticides, when used as seed treatments in these crops, decline rapidly throughout vegetative growth stages. However, the biological impact on target or non-target arthropods was not examined.

Technical Abstract: Neonicotinoids have been implicated as a contributing factor to the observed decreases in honey bee populations. It has been suggested that honey bees can be exposed to seed-treated neonicotinoids through pollen and nectar from treated plants. To investigate the uptake and persistence of neonicotinoids in plant tissue and soil, we conducted seed treatment trials with corn, cotton, and soybean planted in Mississippi, Arkansas, and Tennessee during the 2013 and 2014 growing seasons. Leaf tissue was collected and analyzed beginning shortly after emergence until plants began to flower to better understand how neonicotinoid concentrations change in plant tissues over time. The youngest leaf in the terminal of the plant was sampled as an indicator of the neonicotinoid concentrations within the plant. Soil samples were also collected and analyzed for neonicotinoid concentrations at the first and last sampling dates. The mean clothianidin concentrations in corn treated with Poncho® 250, 500, and 1250 seed treatments declined by 99.3, 99.3, and 97.8 percent, respectively, as the plants developed from seedlings to reproductive plants. The mean concentration of imidacloprid detected in Aeris®-treated cotton decreased by 99.6 percent during the sampling period. For cotton seed treated with Avicta® Duo, the mean concentrations of thiamethoxam and clothianidin in leaf tissue declined by 99.9 and 100 percent, respectively, by the time flowering occurred. There was a 99.9 percent reduction in the mean concentration of thiamethoxam by the time of flowering in leaf tissue from soybean treated with a CruiserMaxx® seed treatment. Mean clothianidin concentrations completely diminished (<1 ng/g) in CruiserMaxx®- and Poncho®/VOTiVO®-treated soybean plants by the time plants reached reproductive growth. The data for neonicotinoid concentrations in the soil were more variable than leaf tissue samples, and the reduction in neonicotinoid concentrations in leaf tissues did not closely correlate with concentrations in the soil. Our results suggest that neonicotinoid insecticides, when used as seed treatments in these crops, decline rapidly throughout vegetative growth stages. However, the biological impact on target or non-target arthropods was not examined.