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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #396127
Research Project: Enhancing Control of Stripe Rusts of Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: A Puccinia striiformis f. sp. tritici effector inhibits high-temperature seedling-plant resistance in wheat

Author
item HU, YANGSHAN - Northwest A&f University
item SU, CHANG - Northwest A&f University
item ZHANG, YUE - Northwest A&f University
item LI, YUXIANG - Northwest A&f University
item Chen, Xianming
item SHANG, HONGSHENG - Northwest A&f University
item HU, XIAOPING - Northwest A&f University

Submitted to: The Plant Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/9/2022
Publication Date: 10/3/2022
Citation: Hu, Y., Su, C., Zhang, Y., Li, Y., Chen, X., Shang, H., Hu, X. 2022. A Puccinia striiformis f. sp. tritici effector inhibits high-temperature seedling-plant resistance in wheat. The Plant Journal. 112(1):249-267. https://doi.org/10.1111/tpj.15945.
DOI: https://doi.org/10.1111/tpj.15945

Interpretive Summary: Resistance to bacterium Pseudomonas syringae pv. maculicola (RPM1)-induced protein kinase (RIPK) in Arabidopsis thaliana belongs to the receptor-like cytoplasmic kinase family and plays a vital role in immunity. Here, we report a similar gene, TaRIPK, in wheat and propose TaRIPK-induced "guard model" during the high-temperature seedling-plant (HTSP) resistance response of wheat to stripe rust. TaRIPK is upregulated during the HTSP resistance response in wheat against the stripe rust pathogen. Knocking down of TaRIPK reduced the expression level of TaRPM1, resulting in a decrease of the HTSP resistance. TaRIPK interacts with and phosphorylates papain-like cysteine protease 1 (TaPLCP1). TaPLCP1 is targeted by PSTG_01766, a secreted protein of the stripe rust pathogen. Overexpressed PSTG_01766 inhibits the basal immunity in tobacco and wheat. PSTG_01766 as an effector protein was further supported by gene silencing and function identification. Thus, TaRIPK positively regulates the HTSP resistance in wheat through induction of TaRPM1 or interaction with the phosphorylation of TaPLCP1, which is also targeted by an effector protein PSTG_01766. Guarded by TaRIPK, TaPLCP1 may serve as a central hub of defence response to stripe rust in wheat. The HTSP resistance appears useful for control of stripe rust.

Technical Abstract: Resistance to Pseudomonas syringae pv. maculicola (RPM1)-induced protein kinase (RIPK) in Arabidopsis thaliana belongs to the receptor-like cytoplasmic kinase family and plays a vital role in immunity. Here, we reported a TaRIPK-induced "guard model" during the high-temperature seedling-plant (HTSP) resistance response of wheat to Puccinia striiformis f. sp. tritici (Pst), the stripe rust pathogen. TaRIPK is upregulated during the HTSP resistance response in wheat against Pst. Knocking down of TaRIPK reduced the expression level of TaRPM1, resulting in a decrease of the HTSP resistance. TaRIPK interacts with and phosphorylates papain-like cysteine protease 1 (TaPLCP1). TaPLCP1 is targeted by PSTG_01766, a secreted protein of Pst. Overexpressed PSTG_01766 inhibits the basal immunity in tobacco and wheat. PSTG_01766 as an effector protein was further supported by gene silencing and function identification. Thus, TaRIPK positively regulates the HTSP resistance in wheat through induction of TaRPM1 or interaction with the phosphorylation of TaPLCP1, which is also targeted by an effector protein PSTG_01766. Guarded by TaRIPK, TaPLCP1 may serve as a central hub of defence response to stripe rust in wheat. The HTSP resistance appears to be an important strategy for controlling stripe rust.