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ARS Home » Southeast Area » Booneville, Arkansas » Dale Bumpers Small Farms Research Center » Research » Publications at this Location » Publication #395744

Research Project: Sustainable Small Farm and Organic Grass and Forage Production Systems for Livestock and Agroforestry

Location: Dale Bumpers Small Farms Research Center

Title: Identification and quantification of bioactive molecules inhibiting pro-inflammatory cytokine production in spent coffee grounds using metabolomics analyses

Author
item HO, KHANH-VAN - University Of Missouri
item SCHREIBER, KATHY - University Of Missouri
item PARK, JIHYUN - University Of Missouri
item VO, PHUC - University Of Missouri
item LEI, ZHENTIAN - University Of Missouri
item SUMNER, LLOYD - University Of Missouri
item BROWN, CHARLES - University Of Missouri
item LIN, CHUNG-HO - University Of Missouri

Submitted to: FRONTIERS IN PHARMACOLOGY
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/19/2020
Publication Date: 3/6/2020
Citation: Ho, K., Schreiber, K.L., Park, J., Vo, P.H., Lei, Z., Sumner, L.W., Brown, C.R., Lin, C. 2020. Identification and quantification of bioactive molecules inhibiting pro-inflammatory cytokine production in spent coffee grounds using metabolomics analyses. Frontiers in Pharmacology. https://doi.org/10.3389/fphar.2020.00229.
DOI: https://doi.org/10.3389/fphar.2020.00229

Interpretive Summary: This study assessed the anti-inflammatory properties of spent coffee grounds. Methanolic extracts of spent coffee grounds obtained from 3 Arabica cultivars possess compounds that exerted inhibitory effects on the secretion of inflammatory mediators. Results indicated that the cytokine suppressive activities of the spent coffee ground (SCG) extracts differed among tested coffee cultivars. The presence of multiple anti-inflammatory compounds in SCGs provides a promising natural source for cosmetic and pharmaceutical industries.

Technical Abstract: In this study, we assessed the anti-inflammatory properties of spent coffee grounds. Methanolic extracts of spent coffee grounds obtained from 3 Arabica cultivars possess compounds that exerted inhibitory effects on the secretion of inflammatory mediators (TNF-a, IL-6, and IL-10) induced by a human pro-monocytic cell line differentiated with PMA and stimulated with lipopolysaccharide (LPS). Our results indicated that the cytokine suppressive activities of the spent coffee ground (SCG) extracts were different among coffee cultivars tested. Hawaiian Kona extracts exhibited inhibitory effects on the expression of 3 examined cytokines, Ethiopian Yirgacheffe extracts reduced the secretion of TNF-a and IL-6, and Costa Rican Tarrazu extracts decreased the secretion of IL-6 only. Untargeted metabolomics analyses of SCG extracts led to the putative identification of 26 metabolites with known anti-inflammatory activities. Multiple metabolites (i.e., chrysin, daidzein, eugenol, naringenin, naringin, oxyresveratrol, pectolinarin, resveratrol, tectochrysin, theaflavin, vanillic acid, and vitexin rhamnoside) identified in the SCGs represent possible novel anti-inflammatory compounds. Of the 26 identified metabolites, the 12 compounds that had high relative intensities in all of the extracts were successfully quantified using liquid chromatography-tandem mass spectrometry analyses. Results from the targeted analyses indicated that caffeine and 5-caffeoylquinic acid (CQA) were the most abundant compounds in the SCG extracts. The contents of caffeine ranged from 0.38 mg/g (Ethiopian Yirgacheffe) – 0.44 mg/g (Costa Rican Tarrazu), whereas 5-CQA concentrations were in the range of 0.24 mg/g (Costa Rican Tarrazu) – 0.34 mg/g (Ethiopian Yirgacheffe). The presence of multiple anti-inflammatory compounds in SCGs provides a promising natural source for cosmetic and pharmaceutical industries.