Location: Location not imported yet.Title: Vesicular stomatitis virus isolated from a bovine brain sample in Costa Rica.
|LEON, BERNAL - National Health Service And Food Quality (SENASA)|
|SANCHEZ, CARLOS - National University|
|CORDERO-SOLORZANO, JUAN - National Health Service And Food Quality (SENASA)|
Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/25/2022
Publication Date: N/A
Interpretive Summary: Vesicular stomatitis virus (VSV) is a insect-transmitted virus that causes vesicular disease in cattle, pigs and horses. The virus, endemic in from northern part of South American, through Central America and in southern Mexico, causes sporadic outbreaks in Western US. Although, in mice VSV invades the brain and symptoms in humans include headaches, there have been no reports of brain infection in farm animals (cattle, pigs and horses). This is the first report and genetic characterization of VSV obtained from the brain of a cow with clinical signs of brain infection(i.e. encephalitis).
Technical Abstract: A retrospective analysis of a brain sample from a bovine with nervous system signs received in SENASA’s laboratory in 2009 was performed. The sample tested negative for rabies by Immunofluorescent direct test and bovine spongiform encephalopathy by histopathology. As part of a study in 2020, the tissue sample was inoculated in Vero E6 cells and the cytopathic effect was evident after 48 hrs. The Vero E6 supernatant was used for RNA extraction and analyzed by new-generation sequencing. The result revealed the presence of Vesicular stomatitis New Jersey virus (VSNJV) which shared (96.10% identity) with the complete genome sequence JX121104, and 96.10% - 96.12% with the glycoprotein sequences M21565 and M21564 respectively. All these sequences belong to New Jersey subtype viruses. This paper aims to report the complete genome sequence isolated in this case.