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ARS Home » Southeast Area » Auburn, Alabama » Soil Dynamics Research » Research » Publications at this Location » Publication #395532
Research Project: GxExM Systems Approach to Crop Disease Management

Location: Soil Dynamics Research

Title: Genome analysis of cotton leafroll dwarf virus reveals variability in the silencing suppressor protein, genotypes and genomic recombinants in the USA

Author
item TABASSUM, AFSHA - University Of Georgia
item BAG, SUDEEP - University Of Georgia
item SUASSUNA, NELSON - Embrapa
item CONNER, KASSIE - Auburn University
item CHEE, PENG - University Of Georgia
item KEMERAIT, ROBERT - University Of Georgia
item ROBERTS, PHILLIP - University Of Georgia

Submitted to: PLOS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/17/2021
Publication Date: 7/7/2021
Citation: Tabassum, A., Bag, S., Suassuna, N.D., Conner, K.N., Chee, P., Kemerait, R.C., Roberts, P. 2021. Genome analysis of cotton leafroll dwarf virus reveals variability in the silencing suppressor protein, genotypes and genomic recombinants in the USA. PLoS ONE. 16(7):e0252523. https://doi.org/10.1371/journal.pone.0252523.
DOI: https://doi.org/10.1371/journal.pone.0252523

Interpretive Summary: Cotton leafroll dwarf virus (CLRDV) is an emerging virus in cotton production in Georgia and several other southeastern states in the USA. Scientists from Univ. of Georgia and Auburn Univ. studied the genetic diversity of the virus population by examining the near complete genome sequences of six isolates from Georgia and one from Alabama. The isolates sequenced were 5,866 nucleotides with seven open reading frames. Isolates from Georgia were >94% identical with other isolates from the USA and South America. Isolates from Georgia and Alabama had a valine, similar to resistant-breaking ‘atypical’, less aggressive genotypes in South America, while the Texas isolate had isoleucine, similar to the more aggressive ‘typical’ genotypes of CLRDV. Ten potential recombinant events were detected in the isolates sequenced. This research will lead to improved understanding of CLRDV population structure and genetic diversity will help develop integrated disease management strategies for CLRDV in the USA cotton belt.

Technical Abstract: Cotton leafroll dwarf virus (CLRDV) is an emerging virus in cotton production in Georgia and several other Southeastern states in the USA. To better understand the genetic diversity of the virus population, the near complete genome sequences of six isolates from Georgia and one from Alabama were determined. The isolates sequenced were 5,866 nucleotides with seven open reading frames (ORFs). The isolates from Georgia were >94% identical with other isolates from the USA and South America. In the silencing suppressor protein (P0), at amino acid position 72, the isolates from Georgia and Alabama had a valine (V), similar to resistant-breaking ‘atypical’ genotypes in South America, while the Texas isolate had isoleucine (I), similar to the more aggressive ‘typical’ genotypes of CLRDV. At position 120, arginine (R) is unique to Georgia and China isolates, but absent in Alabama, Texas and South American isolates. Ten potential recombinant events were detected in the isolates sequenced. An increased understanding of CLRDV population structure and genetic diversity will help develop management strategies for CLRDV in the USA cotton belt.