Location: Sunflower and Plant Biology ResearchTitle: Loci and markers identification associated with freezing tolerant trait in Camelina sativa
|SHAIKH, T - North Dakota State University|
|RAHMAN, MUKHLESUR - North Dakota State University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/4/2022
Publication Date: N/A
Technical Abstract: Lack of freezing tolerance in agronomically important Brassica species limits their use as high yielding, weed-suppressing oilseed crops and cover crops in the Northern Great Plains of the United States and Canada. Two cultivars of Camelina sativa, an emerging oilseed and cover crop, have been identified that show contrasting freezing tolerance after acclimation. Previous studies indicated that freezing tolerance differences between the tolerant variety Joelle and the susceptible variety CO46 was controlled by a few number of dominant or co-dominant genes. Additionally, previously analyzed transcriptome data identified potential regulatory genes that were differentially expressed between these two cultivars and suggested different mechanisms other that CBF and their targets are responsible for freezing tolerance differences. We developed RIL and NIL populations from crosses between these two cultivars and are in process to identify candidate genes underlying these loci. We performed a full genome Illumina sequencing of 28 F3 RILs to ~30X coverage and have sequenced the parental lines to >30X-40X coverage with PacBio HiFi technology along with 60X coverage using Illumina whole genome sequencing. Initial whole genome homozygosity mapping identified ~126k homozygous SNP markers and 64 candidate genes responsible for freezing tolerance difference in these two camelina varieties. However, differentially expressed gene studies during cold acclimation between these two camelina varieties suggests one or more of cysteine-rich RLK genes and receptor serine/threonine kinase genes are primarily responsible for the freezing tolerance differences. We are in process to complete the QTL analysis in our F7 RIL population. Since the mechanism for freezing tolerance differences seems to be novel, we expect our finding will play significant role to improve freezing tolerance in other Brassica crop species and will allow us to develop dual cropping systems in the Northern Great Plains.