Location: Cereal Crops ResearchTitle: Development of genetic tools to study molecular genetics in Xanthomonas translucens
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/13/2022
Publication Date: 8/8/2022
Citation: Schachterle, J.K. 2022. Development of genetic tools to study molecular genetics in Xanthomonas translucens. Meeting Abstract. p.269.
Technical Abstract: Cereal crop species include several of the most important staple crops grown across the globe, and all cereals are hosts for pathogens from the genus Xanthomonas. Barley, oat, and wheat are hosts of X. translucens, a diverse group of Xanthomonads that includes several pathovars. The X. translucens pathovars translucens and undulosa cause bacterial leaf streak in barley and wheat. Although great advances have been made, genetic and molecular understanding of the X. translucens group of pathogens remains limited. One limitation to the advancement of the genetics of X. translucens is that there are few tools available for generating targeted genetic mutations in X. translucens. Several existing tools for such genetic manipulations are not suitable for Xanthomonas species because of limited plasmid host range and the native tolerance of X. translucens to common antibiotics such as ampicillin. To improve upon the available genetic tools effective in X. translucens, we have evaluated strains representing X. translucens pv. translucens and X. translucens pv. undulosa against a panel of antibiotics for suitability in use for genetic selection. We have further constructed a broad host-range plasmid system for the expression of phage recombinase genes under control of an inducible promoter to enable site directed mutagenesis. Our optimization of this expression, transformation, and mutagenesis system will enable further work to study the interactions between X. translucens strains and their hosts.