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ARS Home » Midwest Area » Lexington, Kentucky » Forage-animal Production Research » Research » Publications at this Location » Publication #392936

Research Project: Optimizing the Biology of the Animal-Plant Interface for Improved Sustainability of Forage-Based Animal Enterprises

Location: Forage-animal Production Research

Title: The effect of endophyte-infected tall fescue seed consumption on gut and satiety hormones related to intake in Holstein steers

item KING, MINDY - University Of Kentucky
item HERZING, HANNAH - University Of Kentucky
item MCLEOD, KYLE - University Of Kentucky
item Klotz, James
item HARMON, DAVID - University Of Kentucky

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 4/22/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The objective of this study was to evaluate the impact of endophyte-infected tall fescue (E+) seed, and ergovaline (ERV), on gut and satiety hormones and investigate changes in postabsorptive metabolism. Twelve Holstein steers (260 ± 16 kg) were assigned to one of three treatments: 0 ppm ERV, 1.8 ppm ERV, and 2.7 ppm ERV with animals being adapted to an indoor environment for 4 days followed by a 7-day diet adaptation and a 10-day treatment period. On days 16 and 17, blood was collected via jugular catheters at 20-min intervals for 8 hours beginning 1 hour pre-feeding. Plasma was extracted and analyzed for insulin, leptin, and active ghrelin. DMI decreased linearly with increased ERV concentration (P<0.0001). A quadratic increase was observed for insulin with the 2.7 ppm ERV treatment having the greatest concentrations (P<0.0001). A quadratic response was observed for leptin where the 1.8 ppm ERV treatment had the greatest concentrations (P<0.0001). Active ghrelin concentrations decreased linearly with increasing ERV concentrations (P=0.047). Non-esterified fatty acids (NEFA), '-hydroxybutyrate (BHB), and glucose were analyzed to evaluate changes in postabsorptive metabolism using plasma from each 60-min interval. A treatment-by-time interaction was observed for NEFA (P<0.0001) where the 1.8ppm ERV decreased from pre-feeding to 1-hour post-feeding and did not differ from control thereafter. The 2.7 ppm ERV treatment increased above the 0 ppm ERV treatment beginning 5 hours post-feeding and was greatest among all treatments at 6 hours post-feeding. The 2.7 ppm ERV treatment exhibited the lowest BHB concentrations (P=0.029). Glucose concentrations increased linearly with increasing ERV concentrations (P=0.046). These results indicate that consumption of E+ seed decreases intake which may be possible through alteration of hormones and may also alter postabsorptive metabolism.