|SAMUELS, F - Colorado State University|
|STICH, D - University Of Colorado|
|LEVINGER, N - Colorado State University|
Submitted to: Cryobiology
Publication Type: Abstract Only
Publication Acceptance Date: 7/20/2022
Publication Date: 12/15/2022
Citation: Samuels, F.M., Stich, D.G., Bonnart, R.M., Volk, G.M., Levinger, N.E. 2022. Visualizing PVS2 component permeation into Oryza sativa callus cells by coherent Raman scattering microscopy [abstract]. Cryobiology. 109:38. https://doi.org/10.1016/j.cryobiol.2022.11.121.
Interpretive Summary: N/A
Technical Abstract: It is vital to conserve agricultural and endangered plant species and preserve their genetic diversity. Cryopreservation methods have been developed as be an efficient, long-term method to secure crops and/or species that are at risk in field and greenhouse collections. Plant vitrification solutions 2 and 3 (PVS2 and PVS3) have been used since the 1990s to ensure cell survival after liquid nitrogen exposure. However, these solutions are not universally protective, and it can take years to establish a methodology to successfully cryopreserve a new plant species. This work aims to elucidate how the penetrating cryoprotecting components of PVS2 and PVS3 interact with live Oryza sativa (Asian rice) callus cells. Coherent anti-Stokes Raman scattering (CARS) microscopy enables the direct visualization of deuterated dimethyl sulfoxide, ethylene glycol, and glycerol penetrating live cells. Additionally, monitoring the change in CARS intensity within cells as penetrating CPAs are flowed over the cells gives insight into the rate of penetration of different cryoprotectants.