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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Agricultural Genetic Resources Preservation Research » Research » Publications at this Location » Publication #392672

Research Project: Efficient and Effective Preservation and Management of Plant and Microbial Genetic Resource Collections

Location: Agricultural Genetic Resources Preservation Research

Title: Visualizing PVS2 component permeation into Oryza sativa callus cells by coherent Raman scattering microscopy

item SAMUELS, F - Colorado State University
item STICH, D - University Of Colorado
item Bonnart, Remi
item Volk, Gayle
item LEVINGER, N - Colorado State University

Submitted to: Society for Cryobiology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/20/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary: N/A

Technical Abstract: It is vital to conserve agricultural and endangered plant species and preserve their genetic diversity. Cryopreservation methods have been developed as be an efficient, long-term method to secure crops and/or species that are at risk in field and greenhouse collections. Plant vitrification solutions 2 and 3 (PVS2 and PVS3) have been used since the 1990s to ensure cell survival after liquid nitrogen exposure. However, these solutions are not universally protective, and it can take years to establish a methodology to successfully cryopreserve a new plant species. This work aims to elucidate how the penetrating cryoprotecting components of PVS2 and PVS3 interact with live Oryza sativa (Asian rice) callus cells. Coherent anti-Stokes Raman scattering (CARS) microscopy enables the direct visualization of deuterated dimethyl sulfoxide, ethylene glycol, and glycerol penetrating live cells. Additionally, monitoring the change in CARS intensity within cells as penetrating CPAs are flowed over the cells gives insight into the rate of penetration of different cryoprotectants.