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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #392497
Research Project: Improving Control of Stripe Rusts of Wheat and Barley through Characterization of Pathogen Populations and Enhancement of Host Resistance

Location: Wheat Health, Genetics, and Quality Research

Title: Identification of secreted protein gene-based SNP markers associated with virulence phonotypes of Puccinia striiformis f. sp. tritici, the wheat stripe pathogen

Author
item BAI, QING - Washington State University
item WANG, MEINAN - Washington State University
item XIA, CHONGJING - Washington State University
item See, Deven
item Chen, Xianming

Submitted to: International Journal of Molecular Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/7/2022
Publication Date: 4/8/2022
Citation: Bai, Q., Wang, M., Xia, C., See, D.R., Chen, X. 2022. Identification of secreted protein gene-based SNP markers associated with virulence phonotypes of Puccinia striiformis f. sp. tritici, the wheat stripe pathogen. International Journal of Molecular Sciences. 23(8). Article 4114. https://doi.org/10.3390/ijms23084114.
DOI: https://doi.org/10.3390/ijms23084114

Interpretive Summary: Stripe rust is a destructive disease across major wheat growing regions of the world. This pathogen is highly variable due to the capacity of rapid changes of virulent races circumventing resistance in wheat cultivars and genotypes adapting to different environments. Intensive efforts have been made to study genetics of wheat resistance to this disease, however, no known avirulence genes have been molecularly identified in this fungal pathogen so far. To identify molecular markers for avirulence genes, a Pst panel of 157 selected isolates representing 126 races with diverse virulence spectra was genotyped using 209 secreted protein gene-based single nucleotide polymorphism (SP-SNP) markers using association analysis. Nineteen SP-SNP markers were identified for significant associations with 12 avirulence genes. Some SP-SNPs were associated with two or more avirulence genes. These results further confirmed that association analysis in combination with SP-SNP markers is powerful to identify markers for avirulence genes. This study provides genomic resources for further studies for cloning avirulence genes, understanding mechanisms of host-pathogen interactions, and developing functional markers for tagging specific virulence genes and race groups.

Technical Abstract: Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a destructive disease across major wheat growing regions of the world. This pathogen is highly variable due to the capacity of rapid changes of virulent races circumventing resistance in wheat cultivars and genotypes adapting to different environments. Intensive efforts have been made to study genetics of wheat resistance to this disease, however, no known avirulence genes have been molecularly identified in Pst so far. To identify molecular markers for avirulence genes, a Pst panel of 157 selected isolates representing 126 races with diverse virulence spectra was genotyped using 209 secreted protein gene-based single nucleotide polymorphism (SP-SNP) markers using association analysis. Nineteen SP-SNP markers were identified for significant associations with 12 avirulence genes AvYr1, AvYr6, AvYr7, AvYr9, AvYr10, AvYr24, AvYr27, AvYr32, AvYr43, AvYr44, AvYrSP, and AvYr76. Some SP-SNPs were associated with two or more avirulence genes. These results further confirmed that associ-ation analysis in combination with SP-SNP markers is powerful to identify markers for avirulence genes. This study provides genomic resources for further studies for cloning avirulence genes, understanding mechanisms of host-pathogen interactions, and developing functional markers for tagging specific virulence genes and race groups.