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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #392168

Research Project: Intervention Strategies for Spirochete Diseases

Location: Infectious Bacterial Diseases Research

Title: Leptospira sanjuanensis sp. nov., a pathogenic species of the genus Leptospira isolated from soil in Puerto Rico

Author
item FERNANDES, LUIS - Butantan Institute
item STONE, NATHAN - Northern Arizona University
item ROE, CHANDLER - Northern Arizona University
item GORIS, MARGA - University Medical Center Amsterdam
item VAN DER LINDEN, HANS - University Medical Center Amsterdam
item SAHL, JASON - Northern Arizona University
item WAGNER, DAVID - Northern Arizona University
item Nally, Jarlath

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/28/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary: Leptospirosis is a global zoonotic disease of animals and humans that is caused by pathogenic species of Leptospira. Here, we describe the isolation and characterization of a new pathogenic species of Leptospira from soil samples in Puerto Rico, which was designated L. sanjuanensis. This new species also appears to be a new serovar of Leptospira, which is not currently detected by the currently available serology diagnostic assays.

Technical Abstract: Two spirochetes (designated strains LGVF01 and LGVF02) were isolated from soil samples in San Juan, Puerto Rico in HAN media after selection using a combination of ELISA, agar plating, and colony screening by Fluorescent Antibody Testing (FAT) and PCR for lipL32 and secY. Isolates were helix-shaped, aerobic, fast-growing, and highly motile. Genome sequence analysis indicated that both strains should be classified as members of a novel species within the pathogenic (P1) clade of the genus Leptospira. The average nucleotide identity between the two strains was 99.2%, but below 93.2% when compared to any previously described leptospiral species. Serotyping of strain LGVF02 indicates that it does not belong within any serogroup of Leptospira suggesting it also represents a new serovar. Collectively, strains LGVF01 and LGVF02 represent a new species of pathogenic leptospires for which the name Leptospira sanjuanensis sp. nov. is proposed. The type strain is LGVF02.