Location: Commodity Utilization ResearchTitle: Survey of cottonseed extracts on gene expression in human colon cancer cells
Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Proceedings
Publication Acceptance Date: 4/25/2022
Publication Date: 5/16/2022
Citation: Cao, H., Sethumadhavan, K. 2022. Survey of cottonseed extracts on gene expression in human colon cancer cells. In: Proceedings of the National Cotton Council Beltwide Cotton Conferences, January 4-6, 2022, San Antonio, Texas. p. 478-491.
Technical Abstract: Cotton plant provides economically important fiber and cottonseed, but cottonseed contributes only 20% of the crop value. Cottonseed value could be increased by providing high value bioactive compounds and polyphenolic extracts aimed at improving nutrition and preventing diseases because plant polyphenol extracts have been used as medicinal remedy for various diseases. The objective of this study was to investigate the effects of cottonseed extracts on cell viability and gene expression in mammalian cells. Human colon cancer cells (COLO 225) were treated with ethanol extracts from glanded and glandless cottonseed followed by MTT, qPCR and immunoblotting assays. Cottonseed extracts showed minor effects on cell viability under the experimental conditions. qPCR assay analyzed dozens of mRNAs involved in several important pathways including glucose transport, lipid biosynthesis and inflammation. Cottonseed extracts showed some effects on the expression of genes coding for DGAT, GLUT, TTP, IL, gossypol-regulated and TTP-mediated genes. Glandless seed kernel extract significantly reduced mRNA levels of many genes involved in glucose transport, lipid biosynthesis and inflammation in colon cancer cells. The inhibitory effects of glandless kernel extract on gene expression may provide a useful opportunity for improving nutrition and healthcare associated with colon cancer. This in turn may provide the potential of increasing cottonseed value by using ethanol extract as a nutrition/health intervention agent.