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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #390557

Research Project: Gene Discovery and Crop Design for Current and New Rice Management Practices and Market Opportunities

Location: Dale Bumpers National Rice Research Center

Title: Exploring relationships between phenotypic groups and genotypic subpopulations in ancestral rice, the Oryza rufipogon Species Complex (ORSC)

Author
item Eizenga, Georgia
item KIM, HYUN-JUNG - Cornell University - New York
item JUNG, JANELLE - Cornell University - New York
item MCNALLY, KENNETH - International Rice Research Institute
item GREENBERG, ANTHONY - Bayesic Research
item Edwards, Jeremy
item NAREDO, ELIZABETH - International Rice Research Institute
item BANATICLA-HILARIO, CELESTE - International Rice Research Institute
item HARRINGTON, SANDRA - Cornell University - New York
item SHI, YUXIN - Cornell University - New York
item KIMBALL, JENNIFER - University Of Minnesota
item HARPER, LISA - Cornell University - New York
item MCCOUCH, SUSAN - Cornell University - New York

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/7/2022
Publication Date: 1/7/2022
Citation: Eizenga, G.C., Kim, H., Jung, J., McNally, K.L., Greenberg, A.J., Edwards, J., Naredo, E.B., Banaticla-Hilario, C.N., Harrington, S.E., Shi, Y., Kimball, J.A., Harper, L.A., McCouch, S.R. 2022. Exploring relationships between phenotypic groups and genotypic subpopulations in ancestral rice, the Oryza rufipogon Species Complex (ORSC). Abstract. Plant and Animal Genome Conference. San Diego, California. January 8-12, 2022.

Interpretive Summary:

Technical Abstract: The wild progenitors of Asian cultivated rice (Oryza sativa L.), O. rufipogon and O. nivara, often referred to as the Oryza rufipogon species complex (ORSC), are a valuable reservoir of novel variation for breeding. A collection of 240 diverse ORSC accessions from the International Rice Germplasm Collection, previously characterized by genotyping-by-sequencing (GBS) with 113,739 SNPs, was phenotyped for 44 traits associated with plant, panicle, and seed morphology in the screenhouse at the International Rice Research Institute, Philippines. These traits included heritable phenotypes often recorded as passport data by genebanks. Over 100 of these ORSC accessions were also phenotyped in the greenhouse at Ithaca, New York (16 traits) and Stuttgart, Arkansas, USA (18 traits). We implemented a Bayesian Gaussian mixture model to infer accession groups from a subset of these phenotypic data (32 traits) and ascertained three phenotype-based group assignments. We used concordance between the previously reported six genotypic subpopulations based on GBS and newly reported phenotype-based groups to identify a suite of 16 phenotypic traits that can reliably differentiate the ORSC populations. Furthermore, we identified traits, whether measured in tropical or temperate regions, that discriminated the three groups, thus facilitating genebank management of ORSC collections. These phenotypic groups are associated with species designation, life history and mating habit. Two of the phenotypic groups are consistent with traditional species designations, while one, comprising 20% of our collection, harbored highly admixed accessions with >35% introgression from O. sativa and confounds previous species classification.