Location: Poultry ResearchTitle: Whole-Genome sequencing of three M. gallisepticum isolates similar to the 6/85 vaccine strain
Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/1/2023
Publication Date: 6/22/2023
Citation: Leigh, S.A., Evans, J.D. 2023. Whole-Genome sequencing of three M. gallisepticum isolates similar to the 6/85 vaccine strain. Microbiology Resource Announcements. https://doi.org/10.1128/mra.00339-23.
Interpretive Summary: Mycoplasma gallisepticum field strains with genetic markers identical to vaccine strains are found on a periodic basis. However, the limited genetic sequence data available for comparison has hampered the ability to compare these field isolates to the vaccine strain and determine their origins. Genomic sequencing of three M. gallisepticum was performed and the sequences were compared to the vaccine strain. The results show that the field isolates are highly similar to the vaccine strain with few genetic differences, suggesting that the strains are all related. The results of this work may help in improving current vaccines and development of new diagnostic techniques.
Technical Abstract: Effective control of Mycoplasma gallisepticum (MG) infection has been accomplished through vaccination with live attenuated vaccines. However, virulent MG strains with genetic markers consistent with vaccine strains have been found in infected flocks. We report here the complete genetic sequence of three isolates that are genetically similar to the 6/85 vaccine strain.