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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #388037

Research Project: Intervention Strategies to Control Endemic and New and Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: How transcriptomics can unravel disease pathology

item SARLO DAVILA, KAITLYN - Orise Fellow
item Miller, Laura

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/28/2021
Publication Date: 11/3/2021
Citation: Sarlo Davila, K.M., Miller, L.C. 2021. How transcriptomics can unravel disease pathology. Meeting Abstract. Visions III Conference, Ames,Iowa.

Interpretive Summary:

Technical Abstract: Transcriptomic analysis of mediastinal lymph node from pigs infected with Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), influenza B (IBV), or coinfected with both viruses may indicate why PRRSV infections persist, especially in the lymph nodes, while, IBV infections clear, and provide a greater understanding pathology of these diseases. Differences in gene expression between treatment groups were identified using DeSeq2 and g:Gost on samples from 3 pigs from each treatment group on day 3 post infection. The most significantly (FDR <0.05) over-enriched gene ontology (G.O.) terms were dramatically different between the IBV and PRRSV infected samples. These results demonstrate the key role that transcriptomics may have in the future as a means to unravel disease pathology and unite the disciplines of genomics and veterinary medicine to both breed healthier animals, devise cutting edge interventions for animals affected by disease, and perhaps one day provide personalized medicine in the veterinary field.