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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Mycology and Nematology Genetic Diversity and Biology Laboratory » Research » Publications at this Location » Publication #387786

Research Project: Molecular Systematics, Identification, Biology, and Management of Crop-Parasitic Nematodes

Location: Mycology and Nematology Genetic Diversity and Biology Laboratory

Title: Molecular and morphological characterization of Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 from Egypt

item HAROON, SANAA - El-Fayoum University
item Handoo, Zafar
item KANTOR, MIHAIL - Oak Ridge Institute For Science And Education (ORISE)
item Skantar, Andrea
item Hult, Maria

Submitted to: Notulae Scientia Biologicae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/5/2021
Publication Date: 11/11/2021
Citation: Haroon, S., Handoo, Z.A., Kantor, M.R., Skantar, A.M., Hult, M. 2021. Molecular and morphological characterization of Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 from Egypt. Notulae Scientia Biologicae. 13(4):11083.

Interpretive Summary: Plant-parasitic nematodes are microscopic worms that feed on plants and cause an estimated ten billion dollars of crop losses each year in the United States and 100 billion dollars globally. The cyst nematodes are an important group, with many species that cause significant economic and yield losses on potato. Globodera spp. are the most important nematode pests of potato. In the present study, a high number of cysts were recovered during a field survey in the Abo El Matamer, Egypt. After a careful morphological and molecular examination of cysts and second stage juveniles(J2), we were able to identify the species as Globodera rostochiensis. This report provides new molecular and morphological information obtained on Globodera rostochiensis population from Egypt. The results are important for tracking the spread of this quarantine important nematode. Therefore, this research will be used by scientists and diagnosticians to accurately detect the golden potato cyst nematode from infested fields.

Technical Abstract: The golden potato cyst nematode, Globodera rostochiensis (Wollenweber 1923) Skarbilovich, 1959 is a damaging soilborne quarantine pest of Solanum tuberosum (potato) and other solanaceous crops worldwide. In spring of 2021 a survey was conducted in area of Abo El Matamer, Bahera governorates in Egypt. Soil samples were taken in zigzag pattern throughout 65 acres of potato cultivated land and processed in Nematology lab, Fayoum University, Egypt. In June 2021, two hundred soil samples were collected from nearby areas to evaluate the distribution of this potato cyst nematode in other cultivated land located in area of first infection but fortunately the golden potato cyst nematode was not detected from neighboring locations. The nematode species was identified by both morphological and molecular means as Globodera rostochiensis. To our knowledge this is the first molecular and morphological characterization of G. rostochiensis from Egypt.