|BAGERI, MOTAHAREH - Shiraz University|
|HEIDARI, BAHRAM - Shiraz University|
|DADKHODAIE, ALI - Shiraz University|
|HEIDARI, ZAHRA - Shiraz University|
|DANESHNIA, NEGAR - Shiraz University|
Submitted to: Journal of Crop Science and Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/25/2021
Publication Date: 7/1/2021
Citation: Bageri, M.A., Heidari, B.M., Dadkhodaie, A.M., Heidari, Z., Daneshnia, N., Richards, C.M. 2021. Analysis of genetic diversity in a collection of Plantago species: Application of ISSR markers. Journal of Crop Science and Biotechnology. https://doi.org/10.1007/s12892-021-00107-3.
Interpretive Summary: This study examines the diversity among eight Plantago species collected across Iran. While these data are not sufficient to infer ecological patterns of differentiation in the wild, they are a first step in estimating the range of genetic variation in off-site accessions within this important genus. Thirty one accessions collected from 8 species were DNA genotyped using dominant inter simple sequence repeat markers. The markers were able to cluster most of the 8 species and rank their interspecific diversity. The results suggest that ISSR markers can differentiate Plantago species and that information on genetic diversity may assist in pre-breeding evaluations and Plantago germplasm conservation.
Technical Abstract: The analysis of genetic diversity in medicinal plant species can greatly facilitate germplasm conservation and selection for use in breeding schemes. The aim of the present study was to assess the genetic diversity and differentiation of eight Plantago species using inter simple sequence repeat (ISSR) markers. Thirty-one Plantago accessions belonging to eight species were collected from Iran environments. Polymorphisms in the DNA of Plantago accessions were analyzed using 25 ISSR primers. The data for 286 polymorphic bands indicated that the ISSR primers each amplified 5–21 polymorphic amplicons that were 100–3000 bp in size. The mean polymorphism was 83.83% and five primers showed higher polymorphism. The polymorphism information content (PIC) ranged from 0.1103 to 0.3829 with a mean of 0.2727. Accessions in P. amplexicaulis and P. pysillum represented the highest Nei's and Shannon's genetic diversity, while the lowest was obtained for P. lagopus. The phenetic network generated by the Neighbor-Net algorithm showed moderate conflict and splitting of the eight species. Principal coordinate analysis (PCoA) results showed the separation of accessions of the eight species. The results suggest that ISSR markers can differentiate Plantago species and that information on genetic diversity may assist in pre-breeding evaluations and Plantago germplasm conservation.