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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #386473

Research Project: Intervention Strategies to Control Endemic and New and Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: Host response in the porcine lymph node to infection by PRRSV 2, Influenza B and their coinfection

item SARLO DAVILA, KAITLYN - Orise Fellow
item FLEMING, DAMARIUS - Orise Fellow
item MA, WENJUN - Arkansas State University
item SANG, YONGMING - Tennessee State University
item Miller, Laura

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/23/2021
Publication Date: 12/4/2021
Citation: Sarlo Davila, K.M., Fleming, D.S., Ma, W., Sang, Y., Miller, L.C. 2021. Host response in the porcine lymph node to infection by PRRSV 2, Influenza B and their coinfection. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Both Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and influenza B (IBV) cause natural infections in pigs. PRRSV is the more common swine infection and can develop coinfections with IBV, a zoonotic virus able to infect humans. As a primary infection PRRSV can suppress the host immune system, leaving pigs susceptible to secondary infections such as IBV and contributing to the enormous economic impact of the disease. This study investigates the host transcriptomic response in the lymph node following PRRSV and IBV infections as well as their coinfection. Seronegative pigs 3 to 4 weeks old were split into four treatment groups: control; intranasally infected with Type 2 PRRSV NPB strain; intranasally infected with B/Brisbane/60/2008 virus; or intranasally coinfected with both viruses. Three pigs from each of the four treatment groups were necropsied 5 days post-infection(dpi) and lymph node samples were collected for transcriptomic analysis. Differentially expressed gene (DEG) analysis was carried out using DeSeq2 based on the model treatment + E. The coinfected group had 13 DEGs which were not significant in either independent infection. Many of these genes play a role in the glycerolipid metabolism, membrane transport and MAPK pathways which suggests the creation of a suitable environment for viral replication which may allow the lymph nodes to function as reservoir and establish a persistent infection.