|ABRAM, PAUL - Agriculture And Agri-Food Canada|
|HUEPPELSHEUSER, TRACY - British Colombia Ministry Of Agriculture And Lands|
|FRANKLIN, MICHELLE - Agriculture And Agri-Food Canada|
|DAANE, KENT - University Of California|
|LUE, CHIA-HUA - City University Of New York|
|GIROD, PIERRE - University Of British Columbia|
|CARRILLO, JULI - University Of British Columbia|
|WONG, WARREN - University Of British Columbia|
|MOFFAT, CHANDRA - Agriculture And Agri-Food Canada|
|SIAL, ASHFAQ - University Of Georgia|
Submitted to: Journal of Economic Entomology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/16/2021
Publication Date: 1/4/2022
Citation: Abram, P.K., Wang, X., Hueppelsheuser, T., Franklin, M.R., Daane, K.M., Lee, J.C., Lue, C., Girod, P., Carrillo, J., Wong, W.H., Kula, R.R., Gates, M.W., Hogg, B.N., Moffat, C.E., Hoelmer, K.A., Sial, A., Buffington, M.L. 2022. A coordinated sampling and identification methodology for larval parasitoids of spotted-wing drosophila. Journal of Economic Entomology. https://doi.org/10.1093/jee/toab237.
Interpretive Summary: Spotted-wing drosophila (SWD), native to East Asia, has invaded and widely established in the Americas and Europe, becoming a devastating pest of soft-skinned fruits in its invaded regions. Natural enemies will play a unique role in sustainable area-wide management for this pest. However, standardized sampling methods for estimating the effect of natural enemies in the field are lacking. We provide methodological recommendations for sampling and identifying SWD natural enemies. These methods will be used by biological control practitioners to evaluate the establishment and impact of natural enemies on SWD in the future.
Technical Abstract: We provide recommendations for sampling and identification of introduced larval parasitoids of spotted wing Drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) that are under consideration for classical biological control introductions, or whose adventive (presumed to have been accidentally introduced) populations have recently been discovered in North America and Europe. Within the context of the ecology of D. suzukii and its parasitoids, we discuss advantages and disadvantages of estimating larval parasitism levels using different methods including naturally collected fruit samples and sentinel baits. We then provide recommendations for how to separate Drosophilidae pupae from host fruit material in order to accurately estimate parasitism levels and establish firm host-parasitoid associations, and provide guidelines for identification of emerging parasitoids including a key to the common families of parasitoids of D. suzukii. We anticipate that the guidelines for methodology and interpretation of results that we provide here will form the basis for a large, multi-research group sampling effort in the coming years to characterize the biological control and non-target impacts of accidentally and intentionally introduced larval parasitoids of D. suzukii in several areas of the world.