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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Molecular Plant Pathology Laboratory » Research » Publications at this Location » Publication #383961
Research Project: Development of Novel Control Strategies for Diseases Caused by Cellular and Sub-cellular Pathogens

Location: Molecular Plant Pathology Laboratory

Title: Distribution and genetic variability of natural infection by Mexican papita viroid on tomato fields in Central Mexico

Author
item AVINA-PADILLA, KATIA - Langebio Cinvestav
item MACORRA, ERIKA ZAMORA - University Of Chapingo
item ZUNIGA-ROMANO, MARIA DEL CARME - University Of Chapingo
item AGUIRRE, FLOR ALCANTAR - Langebio Cinvestav
item HERNANDEZ-ROSALES, MARIBEL - Langebio Cinvestav
item Hammond, Rosemarie

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 4/16/2021
Publication Date: 10/20/2021
Citation: Avina-Padilla, K., Macorra, E., Zuniga-Romano, M.N., Aguirre, F., Hernandez-Rosales, M., Hammond, R. 2021. Distribution and genetic variability of natural infection by Mexican papita viroid on tomato fields in Central Mexico. American Phytopathological Society Abstracts. 111/S2.84. https://doi.org/10.1094/PHYTO-111-10-S2.1.
DOI: https://doi.org/10.1094/PHYTO-111-10-S2.1

Interpretive Summary:

Technical Abstract: In 2013, tomato plants (Solanum lycopersicum) grown in fields located in the Jocotitlan locality of Mexico State exhibited remarkable stunting, leaf chlorosis, and reduced fruit number, among other symptoms frequently related to viroid diseases. Diseased samples were collected andbioassays through mechanical inoculation with leaf extracts of samples demonstrated the causal agent's transmissibility to tomato plants under greenhouse conditions. Inoculated plants expressed symptoms that were similar to those on the source plants. RT-PCR assays of RNA samples were positive with expected size product (~170 bp) consistently detected using Bostan et al., 2004 pospiviroid-specific primers in all symptomatic samples or from the mechanically inoculated tomato plants. Analysis of sequences obtained from direct sequencing of amplicons revealed one dominant sequence with 85% identity to Mexican papita viroid (MPVd) isolate VF2 complete sequence reported in Canada infecting greenhouse tomato (GenBank Accession No. FJ824844.1). In follow-up studies, MPVd infections were identified in tomato fields in the Atlacomulco locality of Mexico State during 2017-2018 and in Ixmiquilpan in the state of Hidalgo in 2020. A total collection of 11 isolates were partially sequenced and analyzed with MAFFT software for phylogenetic relationships. The close relationship between the Mexican and the Canadian isolates suggests that MPVd in these two countries may share a common origin.