Location: Endemic Poultry Viral Diseases ResearchTitle: Newcastle disease virus recombinant expressing two foreign genes from the optimal insertion sites for use as a multivalent vaccine vector.
|HE, LEI - Henan University Of Science And Technology|
|ZHANG, ZHENYU - Harbin Veterinary Research Institute|
Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/5/2021
Publication Date: N/A
Technical Abstract: Several strains of Newcastle disease virus (NDV) have been developed as vectors to express a foreign gene (FG) for vaccine and gene therapy purposes. A majority of these NDV vectors express only a single FG or two FGs from suboptimal insertion sites in NDV genome. In the present study, we generated NDV LaSota vaccine strain-based recombinant viruses to express two FGs, green fluorescent protein (GFP) and red fluorescent protein (RFP) genes, from the identified optimal insertion sites through a combination of the independent transcription unit (ITU) and the internal ribosomal entry site (IRES) expression approaches. Biological assessments showed that these recombinant viruses maintained the similar growth kinetics and viral yields in vitro and in vivo with slightly attenuated pathogenicity when compared with their parental viruses. The expression of FGs through the ITU approach was approximately 4-fold more efficient than that through the IRES approach. However, there was no significant difference in the FG expression efficiency between the two-FG viruses and the single-FG viruses through the same expression approach. These results suggest that the NDV LaSota is a safe and promising vector that could efficiently express two FGs from the identified optimal insertions sites in a controllable manner. The ITU tactic could be used for the expression of a higher amount of FG products as a major antigen or factor, whereas the IRES strategy for expression of a lower amount of FG products as a minor antigen or factor to meet the requirement for vaccine or anticancer therapy.