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Research Project: Integrated Research Approaches for Improving Production Efficiency in Rainbow Trout

Location: Cool and Cold Water Aquaculture Research

Title: A long reads-based De novo assembly of the genome of the Arlee homozygous line reveals structural genome variance in rainbow trout

item Gao, Guangtu
item MAGADAN, SUSANA - Universidade De Vigo
item Waldbieser, Geoffrey - Geoff
item YOUNGBLOOD, RAMEY - Mississippi State University
item WHEELER, PAUL - Washington State University
item Scheffler, Brian
item THORGAARD, GARY - Washington State University
item Palti, Yniv

Submitted to: bioRxiv
Publication Type: Pre-print Publication
Publication Acceptance Date: 12/31/2020
Publication Date: 1/1/2021
Citation: Gao, G., Magadan, S., Waldbieser, G.C., Youngblood, R., Wheeler, P., Scheffler, B.E., Thorgaard, G., Palti, Y. 2021. A long reads-based De novo assembly of the genome of the Arlee homozygous line reveals structural genome variance in rainbow trout. bioRxiv.

Interpretive Summary:

Technical Abstract: Currently, there is still a need to improve the contiguity of the rainbow trout reference genome and to use multiple genetic backgrounds that will represent the genetic diversity of this species. The Arlee doubled haploid line was originated from a domesticated hatchery strain that was originally collected from the northern California coast. The Canu pipeline was used to generate the Arlee line genome de-novo assembly from high coverage PacBio long-reads sequence data. The assembly was further improved with Bionano optical maps and Hi-C proximity ligation sequence data to generate 32 major scaffolds corresponding to the karyotype of the Arlee line (2N=64). It is composed of 938 scaffolds with N50 of 39.16 Mb and a total length of 2.33 Gb, of which ~95% was in 32 chromosome sequences with only 438 gaps between contigs and scaffolds. In rainbow trout the haploid chromosome number can vary from 29 to 32. In the Arlee karyotype the haploid chromosome number is 32 because chromosomes Omy04, 14 and 25 are divided into six acrocentric chromosomes. Additional structural variations that were identified in the Arlee genome included the major inversions on chromosomes Omy05 and Omy20 and additional 15 smaller inversions that will require further validation. This is also the first rainbow trout genome assembly that includes a scaffold with the sex-determination gene (sdY) in the chromosome Y sequence. The utility of this genome assembly is demonstrated through the improved annotation of the duplicated genome loci that harbor the IGH genes on chromosomes Omy12 and Omy13.