Location: Renewable Product Technology ResearchTitle: Synthesis and characterization of cellobiose-derived oligosaccharides with bifidogenic activity by glucansucrase E81
|ISPIRLI, HUMEYRA - Bayburt University
|Skory, Christopher - Chris
|DERTLI, ENES - Yildiz Technical University
Submitted to: Food Bioscience
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/27/2021
Publication Date: 9/27/2021
Citation: Ispirli, H., Bowman, M.J., Skory, C.D., Dertli, E. 2021. Synthesis and characterization of cellobiose-derived oligosaccharides with bifidogenic activity by glucansucrase E81. Food Bioscience. 44(Part A). Article 101388. https://doi.org/10.1016/j.fbio.2021.101388.
Interpretive Summary: Prebiotics are non-digestible sugars that can be added to food products to stimulate growth of beneficial microorganisms in the gastrointestinal tract. An effective method for production of new and more active prebiotics is to use glucansucrases, which are enzymes that can convert different sugars to produce distinct rare sugars with desired prebiotic characteristics. In this study, cellobiose and sucrose were modified with glucansucrase and the resulting products were shown to stimulate growth of favorable bacteria, but these products did not stimulate growth of pathogenic bacteria. Cellobiose is a carbohydrate that can be obtained by enzymatic or acidic hydrolysis of agricultural biomass, and sucrose is common table sugar from sugar cane or sugar beets. This research could be valuable in the production of new prebiotics for improved health.
Technical Abstract: Cellobiose-derived oligosaccharides were produced from acceptor reactions with glucansucrase E81 and their prebiotic potentials were determined in this study. The major product was a mixture of trisaccharides formed by the addition of an a-linked glucose to cellobiose at either the 2-O position of the reducing end (86%) glucose or the 6-O position of the non-reducing end glucose (11%) demonstrated by NMR spectroscopy and LC-MS-LTQ analysis. The purified DP 3 cellobiose-derived oligosaccharides were then tested for in vitro human gastric juice and a-amylase digestion and no digestion was observed during the 6 h digestion period. Importantly, DP 3 cellobiose-derived oligosaccharides promoted the growth of Bifidobacteria at high levels demonstrating a Bifidogenic effect and a mild level of proliferation was observed for Lactobacilli suggesting the prebiotic roles of cellobiose-derived oligosaccharides.