Location: Forage and Range ResearchTitle: Scaffolding CLR and CCS assemblies in polyploid grasses
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2020
Publication Date: N/A
Technical Abstract: PacBio long read sequencing has enabled high quality draft sequencing of complex polyploid plants that contain high percentages of repeat regions and are highly heterozygous. We recently used PacBio CLR and CCS methods to sequence a polyhaploid, yet appropriate pentaploid, Kentucky bluegrass genome, and used the Omni-C proximity ligation method to scaffold the raw assemblies. The scaffolding increased the N50 length over the five-fold, and allowed us to model the number of pseudomolecules in the genome. Coverage was over 96%, and gene families previously indiscernible due to redundancy were sorted and characterized.