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ARS Home » Pacific West Area » Logan, Utah » Forage and Range Research » Research » Publications at this Location » Publication #380608

Research Project: Improved Plant Genetic Resources and Methodologies for Rangelands, Pastures, and Turf Landscapes in the Semiarid Western U.S.

Location: Forage and Range Research

Title: Scaffolding CLR and CCS assemblies in polyploid grasses

item Bushman, Shaun
item Robbins, Matthew
item MAUGHAN, JEFFERY - Brigham Young University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2020
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: PacBio long read sequencing has enabled high quality draft sequencing of complex polyploid plants that contain high percentages of repeat regions and are highly heterozygous. We recently used PacBio CLR and CCS methods to sequence a polyhaploid, yet appropriate pentaploid, Kentucky bluegrass genome, and used the Omni-C proximity ligation method to scaffold the raw assemblies. The scaffolding increased the N50 length over the five-fold, and allowed us to model the number of pseudomolecules in the genome. Coverage was over 96%, and gene families previously indiscernible due to redundancy were sorted and characterized.