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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #380156

Research Project: Improvement of Biotic Stress Resistance in Durum and Hard Red Spring Wheat Using Genetics and Genomics

Location: Cereal Crops Research

Title: Identification and molecular mapping of a major QTL on chromosome 2A conferring resistance to Fusarium head blight in emmer wheat

item LENG, YUEQIANG - North Dakota State University
item POUDEL, BIKASH - North Dakota State University
item Bernardo, Amy
item BIAN, RUOLIN - Kansas State University
item KARMACHARYA, ANIL - North Dakota State University
item MULLINS, JOSEPH - North Dakota State University
item BAI, GUIHUA - Kansas State University
item Xu, Steven
item ZHONG, SHAOBIN - North Dakota State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/23/2020
Publication Date: 12/7/2020
Citation: Leng, Y., Poudel, B., Bernardo, A.E., Bian, R., Karmacharya, A., Mullins, J., Bai, G., Xu, S.S., Zhong, S. 2020. Identification and molecular mapping of a major QTL on chromosome 2A conferring resistance to Fusarium head blight in emmer wheat [abstract]. National Fusarium Head Blight Forum. p.7.

Interpretive Summary:

Technical Abstract: Fusarium head blight (FHB), caused by Fusarium graminearum, is one of the most important diseases of durum wheat (Triticum turgidum L. ssp. durum) and common wheat (Triticum aestivum L.) worldwide. Use of resistant wheat cultivars is the most effective and economically sound approach for management of the disease. However, most of durum wheat cultivars currently grown are highly susceptible to FHB and resistant sources in durum wheat are very limited. In the past decades, great efforts have been devoted to screen various tetraploid wheat species for FHB resistance, and some of them have been reported to be resistant to FHB, although the quantitative trait loci (QTL) for FHB resistance are not well characterized. In this study, we aimed to discover new FHB resistance QTL in a population of 186 recombinant inbred lines (RILs) derived from the cross between Divide, an FHB susceptible durum wheat cultivar, and PI 254188, an FHB resistant emmer wheat (Triticum turgidum L. subsp. dicoccum). The RILs along with their parents were evaluated for reactions to FHB in two greenhouse experiments and one field experiment in 2019 and 2020 and genotyped using the genotyping-by-sequencing (GBS) method. A total of 4,476 single nucleotide polymorphism (SNP) markers with normal segregating ratios were identified in the mapping population, and 1,866 unique SNP markers were used to construct a genetic map of 2656.46 cM. QTL analysis using both phenotypic and genotypic data identified one QTL (Qfhb.ndwp-2A-2) on chromosome 2A of PI 254188, which was consistently significant in all greenhouse and field experiments. This QTL explained 23.2, 13.5, and 17.8% of the phenotypic variation in the two greenhouse experiments and one field experiment, respectively. Another QTL (Qfhb.ndwp-5A-3) on chromosome 5A was only detected in the field experiment, which explained 20.3% of the phenotypic variation. Identification of the QTL along with the closely linked SNP markers will facilitate the introgression of the FHB resistance from PI 254188 into durum wheat cultivars through marker-assisted backcrossing.