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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #380138

Research Project: Improving Control of Stripe Rusts of Wheat and Barley through Characterization of Pathogen Populations and Enhancement of Host Resistance

Location: Wheat Health, Genetics, and Quality Research

Title: The RLK protein TaCRK10 activates wheat high-temperature seedling-plant resistance to stripe rust through interacting with TaH2A.1

Author
item WANG, J - NORTHWEST A&F UNIVERSITY
item WANG, J - NORTHWEST A&F UNIVERSITY
item LI, J - NORTHWEST A&F UNIVERSITY
item SHANG, H - NORTHWEST A&F UNIVERSITY
item Chen, Xianming
item HU, X - NORTHWEST A&F UNIVERSITY

Submitted to: Plant Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/22/2021
Publication Date: 9/28/2021
Citation: Wang, J.H., Wang, J.J., Li, J., Shang, H.S., Chen, X., Hu, X.P. 2021. The RLK protein TaCRK10 activates wheat high-temperature seedling-plant resistance to stripe rust through interacting with TaH2A.1. Plant Journal. 108(5):1241-1255. https://doi.org/10.1111/tpj.15513.
DOI: https://doi.org/10.1111/tpj.15513

Interpretive Summary: Plants sense various pathogens and activate immunity responses through receptor like kinases. Cysteine-rich receptor-like kinases (CRKs) are involved in massive transduction pathways upon perception of a pathogen. In the present study, we identified a CRK gene (designated TaCRK10) from wheat variety Xiaoyan 6 (XY6), which harbors high temperature seedling plant (HTSP) resistance to stripe rust. The expression level of TaCRK10 was induced by inoculation with the stripe rust pathogen and high temperature treatment. Knock-down of TaCRK10 by virus induced gene silencing resulted in attenuated wheat HTSP resistance to stripe rust, while overexpression of TaCRK10 in susceptible variety Fielder enhanced resistance under a normal temperature with reactive oxygen species accumulation and defense-related gene expression of the salicylic acid (SA) pathway. Notably, the kinase domain of TaCRK10 was found to interact with a histone variant TaH2A.1 by screening a cDNA library. The full-length of TaCRK10 interacted with TaH2A.1 in yeast, revealed by bimolecular fluorescence complementation and co-immunoprecipitation. Silencing of TaH2A.1 suppressed wheat resistance to stripe rust with the reduction of SA concentration. These results demonstrate that TaCRK10 interacts with TaH2A.1 and plays a positive role in HTSP resistance to stripe rust in XY6 through the SA signal pathway.

Technical Abstract: Plants sense various pathogens and activate immunity responses through receptor like kinases (RLKs). Cysteine-rich receptor-like kinases (CRKs) are involved in massive transduction pathways upon perception of a pathogen. In the present study, we identified a CRK gene (designated TaCRK10) from wheat variety Xiaoyan 6 (XY6), which harbors high temperature seedling plant (HTSP) resistance to Puccinia striiformis f. sp. tritici (Pst), the fungus causing wheat stripe rust. The expression level of TaCRK10 was induced by Pst inoculation and high temperature treatment. Knock-down of TaCRK10 by virus induced gene silencing resulted in attenuated wheat HTSP resistance to Pst, while overexpression of TaCRK10 in susceptible variety Fielder enhanced resistance under a normal temperature with reactive oxygen species (ROS) accumulation and defense-related gene expression of the salicylic acid (SA) pathway. Notably, the kinase domain of TaCRK10 was found to interact with a histone variant TaH2A.1 by screening a cDNA library. The full-length of TaCRK10 interacted with TaH2A.1 in yeast, revealed by bimolecular fluorescence complementation and co-immunoprecipitation. Silencing of TaH2A.1 suppresses wheat resistance to Pst with the reduction of SA concentration. These results demonstrate that TaCRK10 interacts with TaH2A.1 and plays a positive role in HTSP resistance to Pst in XY6 through the SA signal pathway.