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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Healthy Processed Foods Research » Research » Publications at this Location » Publication #379491

Research Project: Prevention of Obesity Related Metabolic Diseases by Bioactive Components of Food Processing Waste Byproducts and Mitigation of Food Allergies

Location: Healthy Processed Foods Research

Title: Cooked black turtle beans ameliorate insulin resistance and restore gut microbiota in C57BL/6J mice on high-fat diets

item TAN, YUQING - Chinese Agricultural University
item Tam, Christina
item MENG, SHI - Chinese Academy Of Agricultural Sciences
item ZHANG, YAN - Mississippi State University
item ALVES, PRISCILA - Non ARS Employee
item Yokoyama, Wallace - Wally

Submitted to: Foods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/19/2021
Publication Date: 7/22/2021
Citation: Tan, Y., Tam, C.C., Meng, S., Zhang, Y., Alves, P., Yokoyama, W.H. 2021. Cooked black turtle beans ameliorate insulin resistance and restore gut microbiota in C57BL/6J mice on high-fat diets. Foods. 10(8). Article 1691.

Interpretive Summary: Beans are generally low in fat, high in dietary fiber, and high in protein. This research shows that black beans reduced blood biomarkers related to diabetes and HOMA-IR an index of insulin resistance. The black bean diet also reduced low density lipoprotein cholesterol or bad cholesterol and triglycerides, both risk factors for cardiovascular disease. The distribution of healthier gut bacteria were also restored by the black bean diet. The research suggests that the consumption of black beans has multiple health benefits.

Technical Abstract: Male C57BL/6J mice were fed high fat (HF) diets supplemented with cooked black turtle beans (HFB) to prevent obesity related insulin resistance. Mice on both the HF and HFB were obese compared to mice fed a lowfat diet. However, although body, liver, and adipose weights of mice fed HFB diet were lower they were not significantly different from HF diet. Plasma LDL and triglyceride concentrations of mice fed HFB diet were 28% and 36.6% lower than those on HF diet. HOMA-IR index of mice fed HFB diet was 87% lower than HF diet. Diabetes related biomarkers GIP, leptin, glucagon and inflammatory cytokines IL-4 and IL-10 were significantly affected by HFB diet. The gut bacterial composition was reshaped by HFB diet compared to HF diet. The results indicated that cooked black turtle bean consumption could ameliorate insulin resistance and restore gut microbiome in HF diet induced obese mice.