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Research Project: Monitoring and Molecular Characterization of Antimicrobial Resistance in Foodborne Bacteria

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Title: Whole genome sequence analysis of multi-drug resistant Enterobacter hormaechei isolated from imported retail shrimp

Author
item INDUGU, NAGARAJU - University Of Pennsylvania
item SHARMA, LAXMI - Florida State University
item Jackson, Charlene
item SINGH, PRASHANT - Florida State University

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/10/2020
Publication Date: 12/10/2020
Citation: Indugu, N., Sharma, L., Jackson, C.R., Singh, P. 2020. Whole genome sequence analysis of multi-drug resistant Enterobacter hormaechei isolated from imported retail shrimp. Microbiology Resource Announcements. 9:e01103-20. https://doi.org/10.1128/MRA.01103-20.
DOI: https://doi.org/10.1128/MRA.01103-20

Interpretive Summary: Enterobacter hormaechei are opportunistic pathogens and are known to cause urinary and respiratory tract infections in humans. Antibiotic resistant strains of E. hormaechei has been previously isolated from fresh produce and seafood samples. In this study, a multi-drug resistant E. hormaechei from raw shrimp collected from a supermarket store located in Atlanta, Georgia was isolated and characterized. Genes and mutations that confer resistance to sulfonamides, quinolones, phenicol/quinolones, trimethoprim, beta-lactamases, phenicols, fosfomycin, tetracycline, and aminoglycosides as well as ten putative plasmids were identified. As shrimp samples are cooked using milder heat treatment, consumption of shrimp harboring multidrug resistant strains can be a possible carrier of resistance genes to human gut microbiota. This information is important to the shrimp industry as changes in processing practices may be necessary to avoid the presence of antibiotic-resistant strains in cooked shrimps.

Technical Abstract: Here, we announce the draft genome sequences of Enterobacter hormaechei 2B-MC1, isolated from a shrimp sample collected from a farmer’s market in Atlanta (GA, USA). The genome of this strain was 661,561-bp long with a 55.3% G'+'C content. The E. hormaechei 2B-MC1 isolate harbored sul1, sul2, qnrA1, oqxB, dfrA23, blaACT, floR, fosA, tet(A), aph(6)-Id, aph(3'')-Ib antibiotic resistance genes.