Genomic resources for Pseudomonas syringae pv. phaseolicola races 5 and 8

Authors: Cooper, Bret; Yang, Ronghui

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/10/2020
Publication Date: 4/7/2021
Citation: Cooper, B., Yang, R. 2021. Genomic resources for Pseudomonas syringae pv. phaseolicola races 5 and 8. Phytopathology. 111:893-895. https://doi.org/10.1094/phyto-10-20-0462-a.
DOI: https://doi.org/10.1094/phyto-10-20-0462-a

Interpretive Summary: Halo blight disease, caused by a bacterium Pseudomonas syringae pv. phaseolicola, reduces harvests of the dry, edible common bean. A model bean plant, PI G19833, is resistant to race 5 of the bacterium but is susceptible to race 8. Because resistance and susceptibility are dictated in part by bacterial genes, we sequenced the DNA genomes of these two races. Annotation of the DNA sequence data identified 5,890 genes for race 5 and 5,919 genes for race 8. Some genes that exist in race 5 did not exist is race 8 and vice-versa. These data will be useful to scientists in the government, at universities, or at private industry who want to study the bacterial genes that cause disease or trigger resistance in beans.

Technical Abstract: Pseudomonas syringae pv. phaseolicola causes halo blight disease on Phaseolus vulgaris. Using a long-read DNA sequencing platform, we deduced the genome sequences for P. syringae pv. phaseolicola races 5 and 8 that have distinguishable avirulent and virulent phenotypes on P. vulgaris PI G19833, a common bean with an annotated genome sequence. The twelve race 5 assemblies comprise two major 4.5 Mb and 1.4 Mb chromosome-like contigs and ten smaller contigs. The four race 8 assemblies comprise a major 6.1 Mb chromosome and 3 smaller contigs. Annotation yielded 5,890 genes for race 5 and 5,919 genes for race 8. These data will enable the discovery of the genetic and proteomic differences between these two races and allow comparisons to others for which genomic information already exists.