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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » Livestock Arthropod Pest Research Unit » Research » Publications at this Location » Publication #378403
Research Project: Integrated Pest Management of Flies of Veterinary Importance

Location: Livestock Arthropod Pest Research Unit

Title: An inexpensive In Vitro blood-feeding system for wild Aedes albopictus (Diptera: Culicidae)

Author
item Osbrink, Weste

Submitted to: Journal of Entomological Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/26/2021
Publication Date: 1/1/2022
Citation: Osbrink, W.L. 2021. An inexpensive In Vitro blood-feeding system for wild Aedes albopictus (Diptera: Culicidae). Journal of Entomological Science. https://doi.org/10.18474/JES21-35.
DOI: https://doi.org/10.18474/JES21-35

Interpretive Summary: An inexpensive system is described which allows for in-vitro feeding of wild Asian tiger mosquitoes. This system allows for a small laboratory with few resources to have an abundance of mosquitoes for quantifying and replicating treatments without the expense of maintaining a rearing room, keeping vertebrates for blood feeding, staying in compliance with IACOC. This system is ideal for feeding mosquitoes systemic insecticide, pathogens, evaluation of repellent, vertical transmission, investigating effects of sublethal treatments or growth regulators, etc., on F1generation, limited only by the imagination of the researcher and not funding.

Technical Abstract: An inexpensive system is described which allows for in-vitro feeding of wild Aedes albopictus (Skuse). This system allows for a small laboratory with few resources to have an abundance of mosquitoes for quantifying and replicating treatments without the expense of maintaining a rearing room, keeping vertebrates for blood feeding, staying in compliance with IACOC. This system is ideal for feeding mosquitoes systemic insecticide, pathogens, evaluation of repellent, vertical transmission, investigating effects of sublethal treatments or growth regulators, etc., on F1generation, limited only by the imagination of the researcher and not funding.