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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #377232

Research Project: Genetic Improvement of Biotic and Abiotic Stress Tolerance and Nutritional Quality in Hard Winter Wheat

Location: Hard Winter Wheat Genetics Research

Title: Identification of two novel Hessian fly resistance genes H35 and H36 in a hard winter wheat line SD06165

item ZHAO, LANFEI - Kansas State University
item ABDELSALA, NADAR RAGAB - Kansas State University
item XU, YUNFENG - Kansas State University
item Chen, Ming-Shun
item FENG, YI - Kansas State University
item KONG, LINGRAN - Shanghai Academy Of Agricultural Sciences
item Bai, Guihua

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/1/2020
Publication Date: 5/20/2020
Citation: Zhao, L., Abdelsala, N., Xu, Y., Chen, M., Feng, Y., Kong, L., Bai, G. 2020. Identification of two novel Hessian fly resistance genes H35 and H36 in a hard winter wheat line SD06165. Journal of Theoretical and Applied Genetics.

Interpretive Summary: Hessian fly (HF) is a destructive insect pest in wheat. To identify new genes for HF resistance, we evaluated resistance in progeny from a cross of wheat experimental lines SD06165 (resistant) and OK05312 (susceptible). We identified two novel resistance genes and designated them H35 and H36. H35 on chromosome arm 3BS showed a major effect on HF resistance and H36 on chromosome arm 7AS showed a minor effect. The effects of the two genes in combination were more than additive. Breeder-friendly DNA markers for the two genes were developed for marker-assisted selection in wheat breeding programs.

Technical Abstract: Hessian fly (HF), Mayetiola destructor (Say), is one of the most destructive pests in wheat (Triticum aestivum L.) worldwide. Growing resistant cultivars is the most effective approach to minimize Hessian fly damage. To identify new quantitative trait loci (QTLs) for HF resistance, a recombinant inbred line (RIL) population was developed by crossing HF resistant wheat line SD06165 to a susceptible line OK05312. The population was genotyped with 1,709 single nucleotide polymorphisms (SNPs) generated from genotyping-by-sequencing (GBS) and phenotyped for HF resistance in greenhouses. Two novel QTLs for HF resistance were identified from SD06165. The major QTL, designated as H35, was closely linked to SNP marker SDOKSNP7679 on chromosome 3BS that explained 23.8% and 36.0% of the phenotypic variations; the minor QTL, designated as H36, was flanked by SNP markers SDOKSNP1618 and SDOKSNP8089 on chromosome 7AS and explained 8.5% and 13.1% of the phenotypic variation in the two experiments. Significant interaction was detected between the two QTLs. Seventeen SNPs that tightly link to H35 and eight SNPs that tightly link to H36 were converted to Kompetitive Allele Specific Polymerase chain reaction (KASP) markers for selecting these QTLs in breeding programs.