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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #376509

Research Project: Intervention Strategies to Control Endemic and New and Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: Too much of a good thing: Antiviral response and tissue damage during respiratory infections in the porcine lung

item FLEMING, DAMARIUS - Orise Fellow
item Miller, Laura
item TIAN, YUN - Tennessee State University
item LI, YONGHAI - Kansas State University
item MA, WENJUN - Kansas State University
item SANG, YONGMING - Tennessee State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/10/2020
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Objective: The antiviral response leads to host protection through expression of interferon-stimulated genes (ISGs) that clear viruses through mRNA degradation and inhibition of transcription, translation and assembly. Additionally, ISG expression causes activation of an antiviral state in nearby cells. Genes highlighted in this study will help with understanding establishment of the antiviral state in relation to tolerance, susceptibility, and lung damage which has implications in treating infections in livestock and humans. Methods: Pigs were split into 4 treatment groups (control, porcine reproductive and respiratory syndrome virus (PRRSV) infected, influenza B virus (FluB) infected, and FluB/PRRSV coinfection). Lung tissue was collected at 3, 5, and 7 days post infection (dpi) for control, PRRSV and FluB/PRRSV coinfection, and 3 and 5 dpi for FluB. Transcriptomic analysis was performed against S.scrofa 11.1 reference. Differential gene expression (DEG) analysis was carried out using DeSeq2 based on the model treatment + dpi + treatment:dpi + E. Downstream analysis examined the interaction of DEGs across time for over-enrichment.