|WANG, DEGUO - Xuchang University|
|WANG, YONGZHEN - Xuchang University|
|ZHANG, MENG - Xuchang University|
Submitted to: Journal of Nutrition and Metabolism
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/19/2020
Publication Date: 6/25/2020
Citation: Wang, D., Wang, Y., Zhang, M., Liu, Y. 2020. Evaluation of a loop-mediated isothermal amplification (LAMP) method for the detection of Salmonella spp. in terms of sensitivity and applicability. Journal of Nutrition and Metabolism. 3(2):5-5. https://doi.org/10.31487/j.JFNM.2020.02.03.
Interpretive Summary: Salmonella species are important food-borne pathogens that cause disease in humans. There is a need to develop sensitive and specific methods to detect Salmonella in food to prevent infections, and one such promising method is the loop-mediated isothermal amplification (LAMP) technique. Two LAMP assays were developed that target DNA sequences that are found on the chromosome of the different Salmonella species. These two LAMP assays are specific for Salmonella, can detect the pathogen at very low levels, and results provided are very reliable. This study provides LAMP assays that can be used for detection of Salmonella in food and other types of samples.
Technical Abstract: Salmonella spp. are important food-borne pathogens that can cause diseases in humans. Many detection methods have been established in Salmonella spp. using loop-mediated isothermal amplification (LAMP) or reverse transcription loop-mediated isothermal amplification (RT-LAMP). The detection limits of these assays varied from 1 CFU/reaction to 104 CFU/reaction, from 100 fg genomic DNA/reaction to 10 pg genomic DNA/reaction, or from 2.0×101 CFU/mL to 107 CFU/mL for food samples. In this study, LAMP assays were developed using genomic DNA for the detection of Salmonella spp. Two sets of LAMP primers were designed using the invA gene and the 16S-23S rRNA intergenic spacer region (ITS) of S. enterica as the target sequences for two LAMP assays. The detection limits of the two methods were respectively 20 pg S. enterica DNA/reaction and 10 pg S. enterica DNA/reaction at the optimized temperature, and the LAMP methods were of high repeatability and specificity for S. enterica detection. This study provides a baseline for the application of LAMP for the detection of food-borne pathogenic bacteria