Location: Aquatic Animal Health ResearchTitle: Aeromonas hydrophila stimulated with deferoxamine mesylate LC-MSMS
|KIRBY, AUGUSTUS - AUBURN UNIVERSITY|
|PEATMAN, ERIC - AUBURN UNIVERSITY|
Submitted to: PRIDE (Proteomics Identification Database)
Publication Type: Database / Dataset
Publication Acceptance Date: 6/8/2020
Publication Date: N/A
Technical Abstract: The Gram-negative bacterium, Aeromonas hydrophila, has been responsible for extensive losses in the catfish industry for over a decade. Due to this impact, there are ongoing efforts to understand the basic mechanisms that contribute to virulent A. hydrophila (vAh) outbreaks. Recent challenge models demonstrated that vAh cultured in the presence of the iron chelating agent deferoxamine mesylate (DFO) were more virulent to channel catfish (Ictalurus punctatus). The current work sought to evaluate growth characteristics and protein expression of vAh after the addition of DFO. Protein identification and expression was determined using a shotgun proteomics approach where replicate samples were analyzed via liquid chromatography mass spectrometry (LC-MSMS). A database of this protein sequencing has been established at the PRIDE repository (https://www.ebi.ac.uk/pride/) accessible under the accession number PXD019631. Both raw image files from the mass spectrometer and search engine results files have been made available to the public.