Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/2/2020
Publication Date: N/A
Interpretive Summary: none
Technical Abstract: The ability of Campylobacter to grow in primary containers incubated aerobically was examined. Ten ml of media composed of (g/L) beef extract, 50; tryptose, 10; soluble starch, 10; sodium bicarbonate, 5.0; sodium lactate, 3.0; agar, 0.5 was added to 25 ml, 12.5 cm2 culture flasks. The media was inoculated with 104 cfu/ml of Campylobacter coli, Campylobacter fetus, Campylobacter jejuni, or Campylobacter lari; and the flasks were closed with plug-sealed caps, vented caps, or vented caps covered with Parafilm laboratory film. The flasks were incubated aerobically at 37C for 48 h. After incubation, number of cfu/ml of Campylobacter in the media was enumerated on a Campylobacter selective agar composed of Blood Agar Base #2 supplemented with 7.0% horse blood and Blaser-Wang antibiotic mixture that was incubated microaerobically at 37C for 48 h. Additionally, the concentration of carbon dioxide in these flasks was measured in parts per million (ppm) with a CO2 Sampling Data Logger. Significant differences in data were determined using GraphPad InStat statistical software. Results indicated that there was a 4.0 to 5.0 log increase in the number of C. coli, C. fetus, C. jejuni, and C. lari recovered from media incubated in the plug-sealed flasks or the flasks with vented caps covered with Parafilm laboratory film. However, significantly fewer Campylobacter were recovered from flasks with vented caps that were not covered with Parafilm, and no C. jejuni or C. lari were recovered from these flasks. Furthermore, there was no significant difference in the growth of the different species of Campylobacter when cultured in same type of container. There were also significantly higher concentrations of CO2 detected in flasks with plug-seal caps or with vented caps covered with Parafilm than in flasks with vented caps. Conclusions indicate that the ability of Campylobacter to grow in containers incubated aerobically was related to the ability of the containers to retain CO2 produced in the flasks. By not requiring the generation of microaerobic atmospheres, utilization of this medium will allow laboratories to simplify procedures for culturing this pathogen by using this medium in sealed containers that can retain CO2.