Location: Fruit and Tree Nut ResearchTitle: Venturia carpophila - Mating Type Idiomorphs and Genetic Diversity on Peach in the Southeastern U.S.A
|YOUNG, CAROLYN - Noble Research Institute|
|BRANNEN, PHILLIP - University Of Georgia|
|ADASKAVEG, JIM - University Of California|
|CHARLTON, NIKKI - Noble Research Institute|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/13/2020
Publication Date: 12/1/2020
Citation: Bock, C.H., Young, C.A., Zhang, M., Chen, C., Brannen, P.M., Adaskaveg, J., Charlton, N.D. 2020. Venturia carpophila - Mating Type Idiomorphs and Genetic Diversity on Peach in the Southeastern U.S.A. Meeting Abstract. Vol 110, No.12S. https://doi.org/10.1094/PHYTO-110-12-S2.1.
Interpretive Summary: Abstract Only.
Technical Abstract: Peach scab (caused by Venturia carpophila) is a major disease affecting peach production in the southeastern U.S.A. We identified the mating type idiomorphs and explored whether they were in equilibrium, and performed preliminary studies on the population genetic diversity using previously developed microsatellite markers. Mating type gene MAT1-1-1 was identified in an available genome sequence, and the MAT1-2-1 gene was subsequently PCR amplified. Mating type loci structures were consistent with those of other Venturia species (V. effusa and V. inaequalis). Primers designed to each of the mating type genes and a reference gene TUB2 were used as a multiplex PCR reaction to screen 81 isolates from populations of V. carpophila in various locations in the eastern U.S.A. The mating type loci were in equilibrium. The 81 isolates were a genetically diverse group, comprising 69 multilocus genotypes. A discriminant analysis of principal components indicated limited differentiation with overlap among populations. Due to small population isolate numbers, the results are considered preliminary. The ability to identify mating types of V. carpophila provides a basis for understanding reproductive methods of the pathogen in different peach production areas and can be a basis for further studies of genetics of the peach scab pathogen.