Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/31/2020
Publication Date: 6/16/2020
Citation: O'Leary, M.L., Burbank, L.P., Krugner, R., Stenger, D.C. 2020. Complete genome sequences of three Xylella fastidiosa subspecies multiplex strains isolated from olive trees in California, USA. Phytopathology. Available: https://doi.org/10.1094/PHYTO-05-20-0167-A.
Interpretive Summary: Xylella fastidiosa subsp. multiplex (Xfm) is a group of plant pathogenic bacteria that cause disease on many agricultural and ornamental hosts. Between 2008-2012, several strains of Xfm were obtained from olive trees grown in Los Angeles, San Diego, and Ventura Counties in California. These strains did not cause severe disease in olive but did produce leaf scorch symptoms in almond. Similar strains of Xfm have been found in Europe, some of which cause almond leaf scorch. Previously, genome sequences were not available for Xfm strains associated with olive in California. This report describes complete genome sequences of three Xfm strains obtained from olive trees grown in California, which will aid genetic comparison of pathogen strains from Europe and North America.
Technical Abstract: Xylella fastidiosa is a xylem-limited bacterial plant pathogen that causes disease on numerous hosts. Additionally, X. fastidiosa may asymptomatically colonize a wide range of plant species. X. fastidiosa subsp. multiplex has been detected in olive (Olea europaea) trees grown in California as well as in Europe. Strains of X. fastidiosa subsp. multiplex isolated from California olive trees are not known to cause disease on olive, although some can induce leaf-scorch symptoms on almond (Prunus dulcis). No genome assemblies currently exist for olive-associated X. fastidiosa subsp. multiplex strains; therefore, a hybrid assembly method was used to generate complete genome sequences for three X. fastidiosa subsp. multiplex strains (Fillmore, LM10, and RH1) isolated from olive trees grown in Ventura and Los Angeles Counties of California.