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ARS Home » Pacific West Area » Pullman, Washington » Animal Disease Research » Research » Publications at this Location » Publication #374586

Research Project: Identification of Host Factors and Immunopathogenesis of Pneumonia in Domestic and Bighorn Sheep

Location: Animal Disease Research

Title: The EDN2 rs110287192 gene polymorphism is associated with paratuberculosis susceptibility in multibreed cattle population

Author
item CINAR, MEHMET ULAS - Erciyes University
item AKYÜZ, BILAL - Erciyes University
item ARSLAN, KORHAN - Erciyes University
item White, Stephen
item NEIBERGS, HOLLY - Washington State University
item GÜMÜSSOY, KADIR SEMIH - Erciyes University

Submitted to: PLoS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/20/2020
Publication Date: 9/3/2020
Citation: Cinar, M., Akyüz, B., Arslan, K., White, S.N., Neibergs, H.L., Gümüssoy, K. 2020. The EDN2 rs110287192 gene polymorphism is associated with paratuberculosis susceptibility in multibreed cattle population. PLoS One. 15(9):e0238631. https://doi.org/10.1371/journal.pone.0238631.
DOI: https://doi.org/10.1371/journal.pone.0238631

Interpretive Summary: Bovine paratuberculosis or Johne's disease is a slow wasting syndrome which is contagious, chronic, and usually fatal. Total costs for Johne's disease are estimated in the hundreds of millions of dollars annually in the U.S. alone. There has been a great need for effective treatments beyond antibiotic therapy, especially since antibiotic treatment has not solved most cases. Host genetic testing could enable selective breeding of cattle with increased natural resistance to Johne's disease, and prior reports have suggested specific genetic variants in and around the EDN2 might be particularly useful in this regard. We tested these variants in a Turkish population of 821 Anatolian and Holstein cattle, and we identified significant association with serologic measures of Johne's disease particularly for rs110287192. These data validate and extend prior findings. Genetic selection for cattle with the GG genotype at rs110287192 may reduce both individual and herd susceptibility to Johne's disease

Technical Abstract: Background Paratuberculosis (pTB), also known as Johne's disease (JD), is a contagious, chronic and granulomatous inflammatory disease of the intestines of ruminants which is caused by Mycobacterium avium subsp. paratuberculosis (MAP) infection, resulting in crucial economic losses worldwide. Since, currently, no effective cure or a vaccine is available for MAP infection, thus it is important to explore the genetic variants that affect the host Mycobacterium avium subsp. paratuberculosis susceptibility. The aim of this study was to analyze a potential association between EDN2 gene mutations (rs110287192, rs109651404 and rs136707411), that modifies susceptibility to pTB. Methods EDN2 rs110287192, rs109651404 and rs136707411 mutations were genotyped in 68 infected and 753 healthy animals from East Anatolian Red crossbred, Anatolian Black crossbred and Holstein breed cattle by using Custom TaqMan SNP Genotyping Assays. For pTB status, serum antibody levels S/P'= 1.0 were assessed in carriers of the different EDN2 genotypes. Results EDN2 rs110287192 mutation showed a significant association with bovine pTB (adj. p < 0.05). For rs110287192 locus, the odd ratios for GG and TG genotypes versus TT genotypes were 1.73; (95% CI = 0.34 -8.59) and 0.53 (95% CI = 0.12 -2.37) respectively, which indicated that proportion of TG heterozygotes were significantly higher in control animals affected as compared to paratuberculosis animals. On the other hand, while rs136707411 mutation showed a suggestive association with pTB status in the examined cattle population; no association was detected between rs109651404 genotypes and pTB status. Conclusion Selecting animals against rs110287192-GG genotype may decrease the risk of paratuberculosis in cattle belongs to Bos taurus species.