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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #373552

Research Project: Identification of Novel Management Strategies for Key Pests and Pathogens of Grapevine with Emphasis on the Xylella Fastidiosa Pathosystem

Location: Crop Diseases, Pests and Genetics Research

Title: Genome sequence resource of ‘Candidatus Liberibacter asiaticus’ strain 9PA from Brazil

item SILVA, P.A. - Fundecitrus - Brazil
item HUANG, J - South China Agricultural Univerisity
item WULFF, N.A. - Fundecitrus - Brazil
item Krugner, Rodrigo
item Chen, Jianchi

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/12/2020
Publication Date: 11/17/2020
Citation: Silva, P., Huang, J., Wulff, N., Krugner, R., Chen, J. 2020. Genome sequence resource of ‘Candidatus Liberibacter asiaticus’ strain 9PA from Brazil. Plant Disease. 105(1):199-201.

Interpretive Summary: Huanglongbing (HLB) is a highly destructive citrus disease associated with infection of ‘Candidatus Liberibacter asiaticus’ (CLas or HLB pathogen). Because the HLB pathogen cannot be cultured in artificial media, research on HLB has been challenging. Using culture-independent technology, whole genome sequences of CLas have been acquired from many countries, but not yet from Brazil. CLas genome sequences provided critical information for HLB management. In this study, the whole genome of CLas strain 9PA originated from a diseased tree in São Paulo, Brazil, was sequenced. The 9PA genome sequence provides information needed for mitigation of HLB in Brazil and worldwide.

Technical Abstract: ‘Candidatus Liberibacter asiaticus’ (CLas), an unculturable alfa-proteobacterium, is associated with citrus Huanglongbing (HLB), a devastating disease threatening citrus production in Brazil and worldwide. In this study, a draft whole genome sequence of CLas strain 9PA obtained from a sweet orange tree (Citrus sinensis L. cv. Pêra) in São Paulo State, Brazil, is reported. The 9PA genome is 1,155,022 bp with G+C content of 36.5%, along with two prophages: P-BSP1-1 (39,767 bp, Type 1) and P-BSP1-3 (31,802 bp, Type 3). This is the first report of a whole genome sequence of CLas from Brazil. The 9PA genome sequence will contribute to the enrichment of current CLas genome resource and to HLB research and control in Brazil and worldwide.