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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Renewable Product Technology Research » Research » Publications at this Location » Publication #373323
Title: Expression of Ethanol Responsive Genes from Lactobacillus buchneri NRRL B-30929

Author
item Liu, Siqing
item Skory, Christopher
item Qureshi, Nasibuddin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/9/2020
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We previously identified numerous proteins associated with ethanol stress response in a Lactobacillus buchneri strain capable of growing in 10% ethanol and have shown that several of these are capable of conferring ethanol tolerance in other bacteria. In the current study, the functional roles of additional ethanol responsive genes are examined to determine if they can increase ethanol tolerance in E. coli host cells. The recombinant strains carrying ethanol responsive genes were subjected to growth analyses in media with and without 4% ethanol. Among the tested, six genes Lbuc_0522 (NADPH-dependent methylglyoxal reductase), Lbuc_0354 (succinate semialdehyde dehydrogenase), Lbuc_1211(threonyl_tRNA synthetase), Lbuc_2051 (nitroreductase), Lbuc_0707 (branched chain amino acid aminotransferase) and Lbuc_1852 (proline-specific peptidase) conferred host cells tolerance to 4% ethanol. Two genes Lbuc_1523 (phage major capsid protein, HK 97 family) and Lbuc_1319 (phosphoglycerate kinase) conferred host cells slight tolerance to 4% ethanol. Four genes Lbuc_0787 encoding fumarylacetoacetate hydrolase, Lbuc_1219 encoding UDP-N-acetylmuramate-L-alanine ligase, Lbuc_0466 encoding ornithine carbamoyltransferase and Lbuc_0858 encoding glycine hydroxymethyltransferase showed no impact on growth in media with 4% ethanol with IPTG induction when compared with E.coli carrying control pET28b plasmid. The expression of two genes Lbuc_1557 (S-layer glycoprotein) and Lbuc_2157 (6-phosphogluconate dehydrogenase) resulted ethanol sensitivity phenotype.