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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #372532

Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Camellia ringspot associated virus 4, a proposed new foveavirus from Camellia japonica

item ZHEN, LUPING - Fujian Agriculture And Forest University
item CHEN, MADELEINE - Oak Ridge Institute For Science And Education (ORISE)
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/11/2020
Publication Date: 5/14/2020
Citation: Zhen, L., Chen, M., Li, R. 2020. Camellia ringspot associated virus 4, a proposed new foveavirus from Camellia japonica. Archives of Virology.

Interpretive Summary: Ornamental camellias (Camellia spp.) are a group of globally important flowering landscape plants. Various diseases with viral symptoms have been reported in camellias for many years. In this study, a new RNA virus was identified in a camellia tree with foliar ringspots using a sensitive sequencing technology. The complete genomic sequence of this virus was determined. Analysis of its genetic information shows that the virus represents a new virus in the foveavirus group. The virus was detected in additional two camellia trees, one each from a public garden and a commercial nursery. This study provides the information necessary to classify the new virus, and to detect it for further study of virus diseases associated with camellias.

Technical Abstract: One large contig with high sequence similarities to Asian Prunus virus 3 was identified by high-throughput sequencing from a camellia tree with ringspot symptom. The complete genome of this new virus was determined to be 8,829 nucleotides long, excluding 3’ poly(A) tail. Its genome organization resembles that of known foveaviruses but contains an additional open reading frame at the 3’ terminal region. Phylogenetic analysis also place this virus with members of the genus Foveavirus in the family Betaflexiviridae in the same subgroup. The virus, which is provisionally named as camellia ringspot associated virus 4, share 50-56% nucleotide sequence identities with other foveaviruses and should represent a new species of the genus.