Submitted to: Plant Cell Tissue and Organ Culture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/3/2020
Publication Date: 5/13/2020
Citation: Bettoni, J.C., Bonnart, R.M., Volk, G.M. 2021. Challenges in implementing plant shoot tip cryopreservation technologies. Plant Cell Tissue and Organ Culture. 144:21-34. https://doi.org/10.1007/s11240-020-01846-x.
Interpretive Summary: Plant shoot tip cryopreservation is a method for processing vegetative propagules that represent specific cultivars for long term storage in liquid nitrogen. This cryopreservation technique may be used to preserve collections in plant genebanks. There are many reports of cryopreservation successes in the literature; however, only a fraction of those methods has been routinely applied for long-term conservation. It can be difficult for laboratories to adopt published methods, because they don't have the infrastructure, technical skills, and/or because the methods may need to be modified for the available plant materials and treatment conditions. This manuscript describes the challenges in implementing shoot tip cryopreservation methods in laboratories and is intended as a resource to share possible reasons why shoot tip cryopreservation methods may be difficult to implement.
Technical Abstract: Cryopreservation is a safe approach for the long-term preservation of plant genetic resources that complements traditional field and in vitro germplasm collections. There are many reports of cryopreservation successes in the literature; however, only a fraction of those methods have been routinely applied for long-term conservation. A common problem is the difficulty of transferring technology and validating protocols from one laboratory to another. There are many critical factors that include the quality of type of plant materials, preculture conditions, cryopreservation method, cooling, storage and warming conditions, and regrowth assessment methods. In addition, adequate facilities and trained personnel are needed. Herein, we present practical aspects involving challenges in implementing plant shoot tip cryopreservation technologies in cryo-banks.USDA is an equal opportunity employer and provider