Chromosomal length reference assembly for Diaphorina citri using single-molecule sequencing and Hi-C proximity ligation with manually curated genes in developmental, structural and immune pathways

Authors: HOSMANI, PRASHANT - Boyce Thompson Institute; FLORES-GONAZLEZ, MIRELLA - Boyce Thompson Institute; SHIPPY, TERESA - Kansas State University; VOSBURG, CHAD - Indian River State College; MASSIMINO, CRISSY - Indian River State College; TANK, WILL - Kansas State University; REYNOLDS, MAX - Indian River State College; TAMAYO, BLESSY - Indian River State College; MILLER, SHERRY - Kansas State University; NORUS, JORDAN - Indian River State College; Hunter, Wayne

Submitted to: bioRxiv
Publication Type: Research Technical Update
Publication Acceptance Date: 12/11/2019
Publication Date: 12/11/2019
Citation: Hosmani, P.S., Flores-Gonazlez, M., Shippy, T., Vosburg, C., Massimino, C., Tank, W., Reynolds, M., Tamayo, B., Miller, S., Norus, J. 2019. Chromosomal length reference assembly for Diaphorina citri using single-molecule sequencing and Hi-C proximity ligation with manually curated genes in developmental, structural and immune pathways. bioRxiv. https://doi.org/10.1101/869685.
DOI: https://doi.org/10.1101/869685

Interpretive Summary: Hemipterans include some of the most important insect pests of agricultural systems and vectors of plant pathogens. The vector, Diaphorina citri (Asian citrus psyllid) belonging to the Psylloidae superfamily, is the primary target of approaches to stop the spread of the pathogenic bacterium Liberibacter asiaticus that causes Huanglongbing. Presented is an improved a new assembly based on long sequencing from Pacific Biosciences, followed by Dovetail Chicago and Illumina, based scaffolding assemblies. The current assembly Diaphorina genome version_3, has 13 chromosomal length scaffolds with a genome size of 475 Megabases. This is the first report of a chromosomal length assembly in the Hemiptera. Gene-targeting technologies like ribonucleic acid interference(RNAi), antisense oligos and other gene editing technologies require accurate annotation of genes as presented to improve development of psyllid population control strategies, to reduce the spread of huanglongbing.

Technical Abstract: Hemipterans include some of the most important insect pests of agricultural systems and vectors of plant pathogens. The vector, Diaphorina citri (Asian citrus psyllid) belonging to the Psylloidae: Superfamily, is the primary target of approaches to stop the spread of the pathogen Candidatus Liberibacter asiaticus that causes Huanglongbing. Presented is an improved a new assembly based on long sequencing from Pacific Biosciences, followed by Dovetail Chicago and Illumina, Hi-C based scaffolding assemblies. The current assembly Diaphorina citri Genome version 3, (Diaci_v3) has 13 chromosomal length scaffolds with a genome size of 475 Megabases. This is the first report of a chromosomal length assembly in the Hemiptera. Gene-targeting technologies like ribonucleic acid interference (RNAi), antisense oligos and other gene editing technologies require accurate annotation of genes as presented to improve development of psyllid population control strategies, to reduce the spread of huanglongbing.