|WANG, MIN-RUI - Northwest A&f University|
|LAMBARDI, MAURIZIO - National Research Council - Italy|
|ENGELMANN, FLORENT - French Research Institute For The Expolotation Of The Sea (IFREMER)|
|PATHIRANA, RANJITH - New Zealand Institute Of Plant & Food Research|
|PANIS, BART - Katholieke University|
|WANG, QIOA-CHUN - Northwest A&f University|
Submitted to: Plant Cell Tissue and Organ Culture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/10/2020
Publication Date: 2/19/2020
Citation: Wang, M., Lambardi, M., Engelmann, F., Pathirana, R., Panis, B., Volk, G.M., Wang, Q. 2020. Advances in cryopreservation of in vitro-derived propagules: Technologies and explant sources. Plant Cell Tissue and Organ Culture. https://doi.org/10.1007/s11240-020-01770-0.
Interpretive Summary: Plant genebanks use shoot tip cryopreservation methods to conserve plant collections of vegetatively-propagated crops that are primarily maintained in the field and greenhouse. This review describes new cryopreservation technologies that many plant genebanks have implemented to secure their collections in liquid nitrogen. Droplet vitrification and cryoplate shoot tip cryopreservation methods are described in detail. In additional, information is also provided about methods that make use of alternative in vitro derived propagules including small leaf square-bearing adventitious buds, stem disc-bearing adventitious buds, and microtubers. Implementation of these new technologies will likely improve the efficiency and options available for the long-term preservation of vegetatively-propagated crops in genebanks.
Technical Abstract: Future improvements in plant breeding are dependent upon having access to novel plant genetic resources that are available in plant genebanks. Many crops that are vegetatively-propagated are maintained as plants in the field or greenhouse, making them vulnerable to biotic and abiotic threats. Increasingly, plant genebanks are using cryopreservation technologies to secure vegetatively-propagated collections at secondary locations. Droplet vitrification and cryo-plate cryopreservation methods have been used to successfully cryopreserve the shoot tips of many plant species. New propagule types, including small leaf square-bearing adventitious buds, stem disc-bearing adventitious buds, and microtubers are alternative explants for use in cryopreservation. This review describes new technologies for in-vitro based cryopreservation systems that have advanced the field plant cryopreservation. Future advances will allow even more diverse germplasm to be successfully preserved in cryobanks.