|Sites Jr, David|
|YAN, ZONG-BU - University Of Arkansas|
Submitted to: Rice Technical Working Group Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/4/2020
Publication Date: 1/6/2021
Citation: Jia, Y., Box, H.N., Jia, M.H., Sites Jr, D.A., Herring, N.D., Yan, Z., Bianco, T.A. 2021. Identification and characterization of rice blast resistance genes in Minghui 63- a restorer line widely used in hybrid rice production. Proceedings of 38th Rice Technical Working Group Meeting, February 24-27, 2020, Orange Beach, Alabama. p 101. Electronic Publication.
Technical Abstract: Significant numbers of rice growers in the Southern US have been growing hybrid rice since 2003. Minghui 63 is one of the most widely used cultivars in hybrid rice production as a fertility restorer by other researchers. The genome of Minghui 63 was recently determined with PacBio long-read and Illumina paired-end sequencing method. In this study, we evaluated Minghui 63 with 7 commonly found US blast races, IB45, IB49, IC17, ID1, IE1, IG1 and IH1 and found that Minghui 63 has different degrees of resistance to the tested blast races. A cross of Minghui63 with the blast susceptible rice variety M202 was made and a recombinant inbred line population was developed consisting of 275 individuals using single seed descent breeding method under greenhouse conditions. This Minghui63/M202 population was genotyped with 156 polymorphic single sequence repeat (SSR) markers and 6, 16, and 3 lines of which were removed due to non-parental allele, heterozygosity and missing data, respectively. A linkage map with 1022.84CM was constructed using QTL mapping and inclusive composite interval mapping (ICIM) software. We are continuing to evaluate the entire population with 3 more blast races and blast resistance genes identified will be presented.