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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #370546

Research Project: Management of Priority Legume, Oilseed, Vegetable, Forage Grass, Sugar, Ornamental, and Medicinal Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: Fingerprinting pea varieties using genes of interest to breeders and extending the variation within the green/yellow cotyledon color gene (I) to wild germplasm

item WEEDEN, NORMAN - Montana State University
item Coyne, Clarice - Clare
item MCPHEE, KEVIN - Montana State University

Submitted to: North American Pulse Improvement Association
Publication Type: Abstract Only
Publication Acceptance Date: 9/15/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: For variety protection applications and quality control purposes DNA fingerprints need to be discriminating, convenient, and reproducible. In pea there exists so much variability and we know so much about the genetics of traits, it seems appropriate to focus on genes (e.g. seed quality, disease resistance, plant habit) that are of particular interest to breeders. We selected 26 such genes or markers for these genes for initial sequencing, with a minimum of 3 genes on each linkage group. Eventually, conveniently amplified sections of 21 of these genes were sequenced on 96 pea varieties. In general, two to five differences (usually Single Nucleotide Polymorphisms) were identified for each gene, and each variety could be easily distinguished by various combinations of genes. We suggest that the use of these sequences will be the most practical method for describing new varieties in variety protection applications. The I gene (green/yellow cotyledon color) gave a relatively normal level of polymorphism within the yellow dry pea germplasm (5 alleles), but exhibited an amazingly high level in wild pea accessions (41 alleles among 50 accessions). We believe this sequence can be used as an internal 'bar code' to identify and compare wild Pisum accessions in germplasm facilities throughout the world.